Conversion of barley SNPs into PCR-based markers using dCAPS method

Detalhes bibliográficos
Autor(a) principal: Shahinnia,Fahimeh
Data de Publicação: 2009
Outros Autores: Sayed-Tabatabaei,Badraldin Ebrahim
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Genetics and Molecular Biology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020
Resumo: Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.
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spelling Conversion of barley SNPs into PCR-based markers using dCAPS methodBarley (Hordeum vulgare L.)genome sequencingmismatched primerrestriction enzymeSNP genotypingMolecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.Sociedade Brasileira de Genética2009-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020Genetics and Molecular Biology v.32 n.3 2009reponame:Genetics and Molecular Biologyinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S1415-47572009005000047info:eu-repo/semantics/openAccessShahinnia,FahimehSayed-Tabatabaei,Badraldin Ebrahimeng2009-08-10T00:00:00Zoai:scielo:S1415-47572009000300020Revistahttp://www.gmb.org.br/ONGhttps://old.scielo.br/oai/scielo-oai.php||editor@gmb.org.br1678-46851415-4757opendoar:2009-08-10T00:00Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)false
dc.title.none.fl_str_mv Conversion of barley SNPs into PCR-based markers using dCAPS method
title Conversion of barley SNPs into PCR-based markers using dCAPS method
spellingShingle Conversion of barley SNPs into PCR-based markers using dCAPS method
Shahinnia,Fahimeh
Barley (Hordeum vulgare L.)
genome sequencing
mismatched primer
restriction enzyme
SNP genotyping
title_short Conversion of barley SNPs into PCR-based markers using dCAPS method
title_full Conversion of barley SNPs into PCR-based markers using dCAPS method
title_fullStr Conversion of barley SNPs into PCR-based markers using dCAPS method
title_full_unstemmed Conversion of barley SNPs into PCR-based markers using dCAPS method
title_sort Conversion of barley SNPs into PCR-based markers using dCAPS method
author Shahinnia,Fahimeh
author_facet Shahinnia,Fahimeh
Sayed-Tabatabaei,Badraldin Ebrahim
author_role author
author2 Sayed-Tabatabaei,Badraldin Ebrahim
author2_role author
dc.contributor.author.fl_str_mv Shahinnia,Fahimeh
Sayed-Tabatabaei,Badraldin Ebrahim
dc.subject.por.fl_str_mv Barley (Hordeum vulgare L.)
genome sequencing
mismatched primer
restriction enzyme
SNP genotyping
topic Barley (Hordeum vulgare L.)
genome sequencing
mismatched primer
restriction enzyme
SNP genotyping
description Molecular genetic research relies heavily on the ability to detect polymorphisms in DNA. Single nucleotide polymorphisms (SNPs) are the most frequent form of DNA variation in the genome. In combination with a PCR assay, the corresponding SNP can be analyzed as a derived cleaved amplified polymorphic sequence (dCAPS) marker. The dCAPS method exploits the well-known specificity of a restriction endonuclease for its recognition site and can be used to virtually detect any SNP. Here, we describe the use of the dCAPS method for detecting single-nucleotide changes by means of a barley EST, CK569932, PCR-based marker.
publishDate 2009
dc.date.none.fl_str_mv 2009-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572009000300020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1415-47572009005000047
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Genética
publisher.none.fl_str_mv Sociedade Brasileira de Genética
dc.source.none.fl_str_mv Genetics and Molecular Biology v.32 n.3 2009
reponame:Genetics and Molecular Biology
instname:Sociedade Brasileira de Genética (SBG)
instacron:SBG
instname_str Sociedade Brasileira de Genética (SBG)
instacron_str SBG
institution SBG
reponame_str Genetics and Molecular Biology
collection Genetics and Molecular Biology
repository.name.fl_str_mv Genetics and Molecular Biology - Sociedade Brasileira de Genética (SBG)
repository.mail.fl_str_mv ||editor@gmb.org.br
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