Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 1997 |
| Outros Autores: | , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Brazilian Journal of Genetics |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400009 |
Resumo: | The <FONT FACE="Symbol">a</font>-globin major genes from diploid and tetraploid Odontophrynus americanus were studied using PCR-based technology. The cloned and sequenced amplified fragments were shown to contain most of the exon II sequences as well as the whole exon III sequence of the <FONT FACE="Symbol">a</font>-globin gene. Unexpectedly, intron 2 was entirely absent in the amplified fragments of both 2n and 4n origin. High conservation was observed among the obtained sequences when compared to corresponding sequences from human and Xenopus laevis origin. The possibility that these sequences might be pseudogenes is raised |
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SBG-2 |
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Brazilian Journal of Genetics |
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Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura)The <FONT FACE="Symbol">a</font>-globin major genes from diploid and tetraploid Odontophrynus americanus were studied using PCR-based technology. The cloned and sequenced amplified fragments were shown to contain most of the exon II sequences as well as the whole exon III sequence of the <FONT FACE="Symbol">a</font>-globin gene. Unexpectedly, intron 2 was entirely absent in the amplified fragments of both 2n and 4n origin. High conservation was observed among the obtained sequences when compared to corresponding sequences from human and Xenopus laevis origin. The possibility that these sequences might be pseudogenes is raisedSociedade Brasileira de Genética1997-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400009Brazilian Journal of Genetics v.20 n.4 1997reponame:Brazilian Journal of Geneticsinstname:Sociedade Brasileira de Genética (SBG)instacron:SBG10.1590/S0100-84551997000400009info:eu-repo/semantics/openAccessAcedo,Maria Dolores P.Paranhos-Baccalà,GláuciaDenoya,Claudio D.Ruiz,Itamar R.G.eng1998-10-06T00:00:00Zoai:scielo:S0100-84551997000400009Revistahttps://www.gmb.org.br/brazilian-journal-of-geneticsONGhttps://old.scielo.br/oai/scielo-oai.phpsede@sgb.org.br || sede@sgb.org.br0100-84550100-8455opendoar:1998-10-06T00:00Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG)false |
| dc.title.none.fl_str_mv |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| title |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| spellingShingle |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) Acedo,Maria Dolores P. |
| title_short |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| title_full |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| title_fullStr |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| title_full_unstemmed |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| title_sort |
Molecular cloning of exons II and III of the a-globin major gene from Odontophrynus americanus 2n and 4n (Amphibia, Anura) |
| author |
Acedo,Maria Dolores P. |
| author_facet |
Acedo,Maria Dolores P. Paranhos-Baccalà,Gláucia Denoya,Claudio D. Ruiz,Itamar R.G. |
| author_role |
author |
| author2 |
Paranhos-Baccalà,Gláucia Denoya,Claudio D. Ruiz,Itamar R.G. |
| author2_role |
author author author |
| dc.contributor.author.fl_str_mv |
Acedo,Maria Dolores P. Paranhos-Baccalà,Gláucia Denoya,Claudio D. Ruiz,Itamar R.G. |
| description |
The <FONT FACE="Symbol">a</font>-globin major genes from diploid and tetraploid Odontophrynus americanus were studied using PCR-based technology. The cloned and sequenced amplified fragments were shown to contain most of the exon II sequences as well as the whole exon III sequence of the <FONT FACE="Symbol">a</font>-globin gene. Unexpectedly, intron 2 was entirely absent in the amplified fragments of both 2n and 4n origin. High conservation was observed among the obtained sequences when compared to corresponding sequences from human and Xenopus laevis origin. The possibility that these sequences might be pseudogenes is raised |
| publishDate |
1997 |
| dc.date.none.fl_str_mv |
1997-12-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400009 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S0100-84551997000400009 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S0100-84551997000400009 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Genética |
| dc.source.none.fl_str_mv |
Brazilian Journal of Genetics v.20 n.4 1997 reponame:Brazilian Journal of Genetics instname:Sociedade Brasileira de Genética (SBG) instacron:SBG |
| instname_str |
Sociedade Brasileira de Genética (SBG) |
| instacron_str |
SBG |
| institution |
SBG |
| reponame_str |
Brazilian Journal of Genetics |
| collection |
Brazilian Journal of Genetics |
| repository.name.fl_str_mv |
Brazilian Journal of Genetics - Sociedade Brasileira de Genética (SBG) |
| repository.mail.fl_str_mv |
sede@sgb.org.br || sede@sgb.org.br |
| _version_ |
1754734879252152320 |