A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants

Detalhes bibliográficos
Autor(a) principal: Bastos,Frederico F.
Data de Publicação: 2022
Outros Autores: Lima,Lin M. de, Tobar,Santiago A. L., Cunha Bastos,Vera L. F., Cunha Bastos,Jayme
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Neotropical ichthyology (Online)
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300217
Resumo: Abstract Functional enzyme assays to detect sublethal poisoning of Neotropical fish are paramount. Accordingly, we assayed a glutathione S-transferase (GST) activity in liver and kidney cytosols from Piaractus mesopotamicus injected with methyl parathion or benzo[a]pyrene using the substrate 1-chloro-2,4-dinitrobenzene (CDNB), which is the usual substrate for assaying a known general activity of GST. Since the most reactive substrate is required to reveal specific changes in enzyme activity, we also used two alternative substrates, 1,2-dichloro-4-nitrobenzene (DCNB) and ethacrynic acid (ETHA). Activities with CDNB or ETHA did not change. However, assays with DCNB showed that methyl parathion caused a decrease in GST activity in the liver on the 24th, 48th and 96th hour after the injection. DCNB also revealed that GST activity in the liver increased seven days after benzo[a]pyrene injection, coming down to normal after fourteen days. Benzo[a]pyrene, but not methyl parathion, increased the activities with DCNB in cytosol from the kidney seven and fourteen days after the injection. Thus, a decreased liver GST activity assayed with DCNB corresponded to contamination of P. mesopotamicus with methyl parathion. The increase of this GST activity in the liver and the kidney correlates to pacu contamination with polycyclic aromatic hydrocarbons.
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spelling A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutantsContaminationFishGSTPiaractus mesopotamicusSerrasalmidaeAbstract Functional enzyme assays to detect sublethal poisoning of Neotropical fish are paramount. Accordingly, we assayed a glutathione S-transferase (GST) activity in liver and kidney cytosols from Piaractus mesopotamicus injected with methyl parathion or benzo[a]pyrene using the substrate 1-chloro-2,4-dinitrobenzene (CDNB), which is the usual substrate for assaying a known general activity of GST. Since the most reactive substrate is required to reveal specific changes in enzyme activity, we also used two alternative substrates, 1,2-dichloro-4-nitrobenzene (DCNB) and ethacrynic acid (ETHA). Activities with CDNB or ETHA did not change. However, assays with DCNB showed that methyl parathion caused a decrease in GST activity in the liver on the 24th, 48th and 96th hour after the injection. DCNB also revealed that GST activity in the liver increased seven days after benzo[a]pyrene injection, coming down to normal after fourteen days. Benzo[a]pyrene, but not methyl parathion, increased the activities with DCNB in cytosol from the kidney seven and fourteen days after the injection. Thus, a decreased liver GST activity assayed with DCNB corresponded to contamination of P. mesopotamicus with methyl parathion. The increase of this GST activity in the liver and the kidney correlates to pacu contamination with polycyclic aromatic hydrocarbons.Sociedade Brasileira de Ictiologia2022-01-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300217Neotropical Ichthyology v.20 n.3 2022reponame:Neotropical ichthyology (Online)instname:Sociedade Brasileira de Ictiologia (SBI)instacron:SBI10.1590/1982-0224-2022-0025info:eu-repo/semantics/openAccessBastos,Frederico F.Lima,Lin M. deTobar,Santiago A. L.Cunha Bastos,Vera L. F.Cunha Bastos,Jaymeeng2022-11-22T00:00:00Zoai:scielo:S1679-62252022000300217Revistahttp://www.ufrgs.br/ni/https://old.scielo.br/oai/scielo-oai.php||neoichth@nupelia.uem.br1982-02241679-6225opendoar:2022-11-22T00:00Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)false
dc.title.none.fl_str_mv A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
title A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
spellingShingle A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
Bastos,Frederico F.
Contamination
Fish
GST
Piaractus mesopotamicus
Serrasalmidae
title_short A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
title_full A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
title_fullStr A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
title_full_unstemmed A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
title_sort A typical enzyme activity for glutathione conjugation indicates exposure of pacu to pollutants
author Bastos,Frederico F.
author_facet Bastos,Frederico F.
Lima,Lin M. de
Tobar,Santiago A. L.
Cunha Bastos,Vera L. F.
Cunha Bastos,Jayme
author_role author
author2 Lima,Lin M. de
Tobar,Santiago A. L.
Cunha Bastos,Vera L. F.
Cunha Bastos,Jayme
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Bastos,Frederico F.
Lima,Lin M. de
Tobar,Santiago A. L.
Cunha Bastos,Vera L. F.
Cunha Bastos,Jayme
dc.subject.por.fl_str_mv Contamination
Fish
GST
Piaractus mesopotamicus
Serrasalmidae
topic Contamination
Fish
GST
Piaractus mesopotamicus
Serrasalmidae
description Abstract Functional enzyme assays to detect sublethal poisoning of Neotropical fish are paramount. Accordingly, we assayed a glutathione S-transferase (GST) activity in liver and kidney cytosols from Piaractus mesopotamicus injected with methyl parathion or benzo[a]pyrene using the substrate 1-chloro-2,4-dinitrobenzene (CDNB), which is the usual substrate for assaying a known general activity of GST. Since the most reactive substrate is required to reveal specific changes in enzyme activity, we also used two alternative substrates, 1,2-dichloro-4-nitrobenzene (DCNB) and ethacrynic acid (ETHA). Activities with CDNB or ETHA did not change. However, assays with DCNB showed that methyl parathion caused a decrease in GST activity in the liver on the 24th, 48th and 96th hour after the injection. DCNB also revealed that GST activity in the liver increased seven days after benzo[a]pyrene injection, coming down to normal after fourteen days. Benzo[a]pyrene, but not methyl parathion, increased the activities with DCNB in cytosol from the kidney seven and fourteen days after the injection. Thus, a decreased liver GST activity assayed with DCNB corresponded to contamination of P. mesopotamicus with methyl parathion. The increase of this GST activity in the liver and the kidney correlates to pacu contamination with polycyclic aromatic hydrocarbons.
publishDate 2022
dc.date.none.fl_str_mv 2022-01-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300217
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252022000300217
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/1982-0224-2022-0025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
publisher.none.fl_str_mv Sociedade Brasileira de Ictiologia
dc.source.none.fl_str_mv Neotropical Ichthyology v.20 n.3 2022
reponame:Neotropical ichthyology (Online)
instname:Sociedade Brasileira de Ictiologia (SBI)
instacron:SBI
instname_str Sociedade Brasileira de Ictiologia (SBI)
instacron_str SBI
institution SBI
reponame_str Neotropical ichthyology (Online)
collection Neotropical ichthyology (Online)
repository.name.fl_str_mv Neotropical ichthyology (Online) - Sociedade Brasileira de Ictiologia (SBI)
repository.mail.fl_str_mv ||neoichth@nupelia.uem.br
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