Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization
Autor(a) principal: | |
---|---|
Data de Publicação: | 2010 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100026 |
Resumo: | This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together. |
id |
SBM-1_01c8f5a6989d3bb07a932a25425da91e |
---|---|
oai_identifier_str |
oai:scielo:S1517-83822010000100026 |
network_acronym_str |
SBM-1 |
network_name_str |
Brazilian Journal of Microbiology |
repository_id_str |
|
spelling |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterizationBacillus licheniformisproteaselipaseconcomitant productionpurificationThis study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together.Sociedade Brasileira de Microbiologia2010-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100026Brazilian Journal of Microbiology v.41 n.1 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000100026info:eu-repo/semantics/openAccessSangeetha,R.Geetha,A.Arulpandi,I.eng2009-11-23T00:00:00Zoai:scielo:S1517-83822010000100026Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-11-23T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
title |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
spellingShingle |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization Sangeetha,R. Bacillus licheniformis protease lipase concomitant production purification |
title_short |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
title_full |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
title_fullStr |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
title_full_unstemmed |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
title_sort |
Concomitant production of protease and lipase by Bacillus licheniformis VSG1: production, purification and characterization |
author |
Sangeetha,R. |
author_facet |
Sangeetha,R. Geetha,A. Arulpandi,I. |
author_role |
author |
author2 |
Geetha,A. Arulpandi,I. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Sangeetha,R. Geetha,A. Arulpandi,I. |
dc.subject.por.fl_str_mv |
Bacillus licheniformis protease lipase concomitant production purification |
topic |
Bacillus licheniformis protease lipase concomitant production purification |
description |
This study was aimed at producing protease and lipase simultaneously on a common medium by Bacillus licheniformis VSG1, which was isolated from a tannery effluent. The effect of media composition with respect to protein source, lipid source and emulsifier on the production of protease and lipase was analysed. Both those enzymes were produced under optimized conditions like pH, temperature and incubation time. The enzyme mixture comprising of both protease and lipase was purified by ammonium sulphate precipitation, dialysis and gel filtration chromatography to obtain 20-fold pure enzymes. The purified enzyme mixture was characterized to determine the optimum pH and temperature of protease and lipase, the response of the enzymes to inhibitors, additives and solvents. The molecular weight of both the enzymes was determined as 40 kDa on SDS-PAGE. The concomitant production of protease and lipase and the purification of both the enzymes in a single mixture have industrial significance, as many industrial processes use both protease and lipase together. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100026 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000100026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822010000100026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.41 n.1 2010 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122202711392256 |