Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle

Detalhes bibliográficos
Autor(a) principal: Lilenbaum,Walter
Data de Publicação: 2007
Outros Autores: Ferreira,Rachel, Marassi,Carla Dray, Ristow,Paula, Oelemann,Walter Martins Roland, Fonseca,Leila de Souza
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016
Resumo: Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that natural occurring TB strongly interferes in the diagnosis of PTB and that commercial ELISAs cannot be considered reliable tools in the diagnosis of paratuberculosis in tuberculosis-infected herds.
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spelling Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattleParatuberculosisTuberculosisMycobacterium bovisMycobacterium avium paratuberculosisForty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that natural occurring TB strongly interferes in the diagnosis of PTB and that commercial ELISAs cannot be considered reliable tools in the diagnosis of paratuberculosis in tuberculosis-infected herds.Sociedade Brasileira de Microbiologia2007-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016Brazilian Journal of Microbiology v.38 n.3 2007reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822007000300016info:eu-repo/semantics/openAccessLilenbaum,WalterFerreira,RachelMarassi,Carla DrayRistow,PaulaOelemann,Walter Martins RolandFonseca,Leila de Souzaeng2007-10-17T00:00:00Zoai:scielo:S1517-83822007000300016Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2007-10-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
title Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
spellingShingle Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
Lilenbaum,Walter
Paratuberculosis
Tuberculosis
Mycobacterium bovis
Mycobacterium avium paratuberculosis
title_short Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
title_full Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
title_fullStr Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
title_full_unstemmed Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
title_sort Interference of tuberculosis on the performance of ELISAs used in the diagnosis of paratuberculosis in cattle
author Lilenbaum,Walter
author_facet Lilenbaum,Walter
Ferreira,Rachel
Marassi,Carla Dray
Ristow,Paula
Oelemann,Walter Martins Roland
Fonseca,Leila de Souza
author_role author
author2 Ferreira,Rachel
Marassi,Carla Dray
Ristow,Paula
Oelemann,Walter Martins Roland
Fonseca,Leila de Souza
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Lilenbaum,Walter
Ferreira,Rachel
Marassi,Carla Dray
Ristow,Paula
Oelemann,Walter Martins Roland
Fonseca,Leila de Souza
dc.subject.por.fl_str_mv Paratuberculosis
Tuberculosis
Mycobacterium bovis
Mycobacterium avium paratuberculosis
topic Paratuberculosis
Tuberculosis
Mycobacterium bovis
Mycobacterium avium paratuberculosis
description Forty-four cows from five herds infected with tuberculosis (TB) and without paratuberculosis (PTB), and 21 cows from a herd without either infection were studied. The cattle presented concordant results in both the skin test and gamma-interferon assay for TB and two commercial ELISAs for PTB. Animals were divided according to TB test results into Group A with 28 TB-infected animals, Group B with 16 TB-negative animals from infected herds, and Group C with 21 TB-negative cows from a tuberculosis-free herd (which were used as controls). Twenty of 28 animals from Group A (71.4%), 6/16 from Group B (37.5%) and none from Group C were reactive to PTB ELISAs, suggesting that these commercial kits were unable to distinguish between PTB and TB. It is proposed that natural occurring TB strongly interferes in the diagnosis of PTB and that commercial ELISAs cannot be considered reliable tools in the diagnosis of paratuberculosis in tuberculosis-infected herds.
publishDate 2007
dc.date.none.fl_str_mv 2007-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000300016
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822007000300016
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.38 n.3 2007
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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