Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018 |
Resumo: | An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers' intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products. |
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Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, ArgentinaStaphylococcus aureusenterotoxigenic strainsfruit saladsantimicrobial compoundsmultiplex PCRAn increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers' intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products.Sociedade Brasileira de Microbiologia2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018Brazilian Journal of Microbiology v.44 n.4 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014005000001info:eu-repo/semantics/openAccessLucero Estrada,Cecilia S.M.Alcaráz,Lucia E.Satorres,Sara E.Manfredi,EduardoVelázquez,Lidia del C.eng2014-03-27T00:00:00Zoai:scielo:S1517-83822013000400018Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-03-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
title |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
spellingShingle |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina Lucero Estrada,Cecilia S.M. Staphylococcus aureus enterotoxigenic strains fruit salads antimicrobial compounds multiplex PCR |
title_short |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
title_full |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
title_fullStr |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
title_full_unstemmed |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
title_sort |
Presence of enterotoxigenic Staphylococcus aureus in artisan fruit salads in the city of San Luis, Argentina |
author |
Lucero Estrada,Cecilia S.M. |
author_facet |
Lucero Estrada,Cecilia S.M. Alcaráz,Lucia E. Satorres,Sara E. Manfredi,Eduardo Velázquez,Lidia del C. |
author_role |
author |
author2 |
Alcaráz,Lucia E. Satorres,Sara E. Manfredi,Eduardo Velázquez,Lidia del C. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Lucero Estrada,Cecilia S.M. Alcaráz,Lucia E. Satorres,Sara E. Manfredi,Eduardo Velázquez,Lidia del C. |
dc.subject.por.fl_str_mv |
Staphylococcus aureus enterotoxigenic strains fruit salads antimicrobial compounds multiplex PCR |
topic |
Staphylococcus aureus enterotoxigenic strains fruit salads antimicrobial compounds multiplex PCR |
description |
An increase in the consumption of fruit juices and minimally processed fruits salads has been observed in recent years all over the world. In this work, the microbiological quality of artisan fruit salads was analysed. Faecal coliforms, Salmonella spp, Shigella spp, Yersinia enterocolitica and Escherichia coli O157:H7 were not detected; nevertheless, eleven strains of Staphylococcus aureus were isolated. By multiplex PCR, all isolates showed positive results for S. aureus 16S rRNA gene and 63.6% of them were positive for sea gene. Furthermore, PCR sea positive strains were able to produce the corresponding enterotoxin. Finally, the inactivation of these strains in fruit salads by nisin, lysozyme and EDTA, was studied. EDTA produced a total S. aureus growth inhibition after 60 h of incubation at a concentration of 250 mg/L. The presence of S. aureus might indicate inadequate hygiene conditions during salad elaboration; however, the enterotoxigenicity of the strains isolated in this study, highlights the risk of consumers' intoxication. EDTA could be used to inhibit the growth of S. aureus in artisan fruit salads and extend the shelf life of these products. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400018 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822014005000001 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.4 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122207017893888 |