Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil

Detalhes bibliográficos
Autor(a) principal: Knöbl,Terezinha
Data de Publicação: 2006
Outros Autores: Gomes,Tânia Aparecida Tardelli, Vieira,Mônica Aparecida Midolli, Ferreira,Fernando, Bottino,José Américo, Ferreira,Antônio José Piantino
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033
Resumo: Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae probes. The adherence to chicken tracheal cells showed 96% positive strains, while hemagglutination assays showed 26.5% of the isolates were mannose sensitive and 21.7% were mannose resistant.
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spelling Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in BrazilAPECEscherichia coliadhesinscolibacillosispoultryThree hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae probes. The adherence to chicken tracheal cells showed 96% positive strains, while hemagglutination assays showed 26.5% of the isolates were mannose sensitive and 21.7% were mannose resistant.Sociedade Brasileira de Microbiologia2006-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033Brazilian Journal of Microbiology v.37 n.3 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000300033info:eu-repo/semantics/openAccessKnöbl,TerezinhaGomes,Tânia Aparecida TardelliVieira,Mônica Aparecida MidolliFerreira,FernandoBottino,José AméricoFerreira,Antônio José Piantinoeng2006-12-18T00:00:00Zoai:scielo:S1517-83822006000300033Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-12-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
title Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
spellingShingle Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
Knöbl,Terezinha
APEC
Escherichia coli
adhesins
colibacillosis
poultry
title_short Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
title_full Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
title_fullStr Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
title_full_unstemmed Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
title_sort Some adhesins of avian pathogenic Escherichia coli (APEC) isolated from septicemic poultry in Brazil
author Knöbl,Terezinha
author_facet Knöbl,Terezinha
Gomes,Tânia Aparecida Tardelli
Vieira,Mônica Aparecida Midolli
Ferreira,Fernando
Bottino,José Américo
Ferreira,Antônio José Piantino
author_role author
author2 Gomes,Tânia Aparecida Tardelli
Vieira,Mônica Aparecida Midolli
Ferreira,Fernando
Bottino,José Américo
Ferreira,Antônio José Piantino
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Knöbl,Terezinha
Gomes,Tânia Aparecida Tardelli
Vieira,Mônica Aparecida Midolli
Ferreira,Fernando
Bottino,José Américo
Ferreira,Antônio José Piantino
dc.subject.por.fl_str_mv APEC
Escherichia coli
adhesins
colibacillosis
poultry
topic APEC
Escherichia coli
adhesins
colibacillosis
poultry
description Three hundred and fifty strains of E. coli isolated from septicemic poultry from seven states of Brazil were examined for presence of nine adhesion-encoding genes, hemagglutination and adherence to chicken tracheal cells (in vitro). Analysis of the strains by colony hybridization tests demonstrated that 93.7% of the isolates were fim +, 17% pap+ and 5.7% were sfa+. The mannose sensitive fimbriae occur with similar frequency in APEC isolated from all Brazilians states, while significant differences among pap and sfa genes distributions were observed. The results showed that 0.85% and 0.28% of APEC were positive for genes that encoded enteroaggregative adhesins and EPEC adherence factor, respectively. None of APEC was positive for DA, afa, Bfp and Eae probes. The adherence to chicken tracheal cells showed 96% positive strains, while hemagglutination assays showed 26.5% of the isolates were mannose sensitive and 21.7% were mannose resistant.
publishDate 2006
dc.date.none.fl_str_mv 2006-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000300033
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822006000300033
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.37 n.3 2006
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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