New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli

Detalhes bibliográficos
Autor(a) principal: Xavier,Mauro Aparecido Souza
Data de Publicação: 2009
Outros Autores: Kipnis,André, Torres,Fernando Araripe Gonçalves, Astofi-Filho,Spartaco
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400007
Resumo: We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
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spelling New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coliHeterologous expressionEscherichia colimolecular cloninginduced expressionWe report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.Sociedade Brasileira de Microbiologia2009-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400007Brazilian Journal of Microbiology v.40 n.4 2009reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822009000400007info:eu-repo/semantics/openAccessXavier,Mauro Aparecido SouzaKipnis,AndréTorres,Fernando Araripe GonçalvesAstofi-Filho,Spartacoeng2009-10-06T00:00:00Zoai:scielo:S1517-83822009000400007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-10-06T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
title New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
spellingShingle New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
Xavier,Mauro Aparecido Souza
Heterologous expression
Escherichia coli
molecular cloning
induced expression
title_short New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
title_full New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
title_fullStr New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
title_full_unstemmed New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
title_sort New vectors derives from pUC 18 for clonig and thermal-induced expression in Escherichia coli
author Xavier,Mauro Aparecido Souza
author_facet Xavier,Mauro Aparecido Souza
Kipnis,André
Torres,Fernando Araripe Gonçalves
Astofi-Filho,Spartaco
author_role author
author2 Kipnis,André
Torres,Fernando Araripe Gonçalves
Astofi-Filho,Spartaco
author2_role author
author
author
dc.contributor.author.fl_str_mv Xavier,Mauro Aparecido Souza
Kipnis,André
Torres,Fernando Araripe Gonçalves
Astofi-Filho,Spartaco
dc.subject.por.fl_str_mv Heterologous expression
Escherichia coli
molecular cloning
induced expression
topic Heterologous expression
Escherichia coli
molecular cloning
induced expression
description We report the construction of two vectors for Escherichia coli: pUC72, for molecular cloning, and pPLT7, for thermal-induced expression. The main feature of pUC72 is a novel polylinker region that includes restriction sites for Nde I and Nco I which provide an ATG codon for proper translation initiation of expressed genes. Vector pPLT7 is ideal for thermo-inducible expression in host cells that carry the cI857 repressor gene. The use of pPLT7 was validated by the successful expression of the genes encoding carp and porcine growth hormones. These vectors provide novel cloning possibilities in addition to simple, non-expensive, high level expression of recombinant proteins in E. coli.
publishDate 2009
dc.date.none.fl_str_mv 2009-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400007
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000400007
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822009000400007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.40 n.4 2009
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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