Molecular characterization of the first leptospires isolated from goats in Brazil

Detalhes bibliográficos
Autor(a) principal: Lilenbaum,Walter
Data de Publicação: 2014
Outros Autores: Kremer,Frederico, Ristow,Paula, Dellagostin,Odir, Bourhy,Pascale, Hartskeerl,Rudy, Vasconcellos,Silvio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050
Resumo: Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.
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spelling Molecular characterization of the first leptospires isolated from goats in BrazilLeptospiraserogroupingsequencingPFGErrsTwo Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.Sociedade Brasileira de Microbiologia2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050Brazilian Journal of Microbiology v.45 n.4 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000400050info:eu-repo/semantics/openAccessLilenbaum,WalterKremer,FredericoRistow,PaulaDellagostin,OdirBourhy,PascaleHartskeerl,RudyVasconcellos,Silvioeng2015-02-13T00:00:00Zoai:scielo:S1517-83822014000400050Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-02-13T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Molecular characterization of the first leptospires isolated from goats in Brazil
title Molecular characterization of the first leptospires isolated from goats in Brazil
spellingShingle Molecular characterization of the first leptospires isolated from goats in Brazil
Lilenbaum,Walter
Leptospira
serogrouping
sequencing
PFGE
rrs
title_short Molecular characterization of the first leptospires isolated from goats in Brazil
title_full Molecular characterization of the first leptospires isolated from goats in Brazil
title_fullStr Molecular characterization of the first leptospires isolated from goats in Brazil
title_full_unstemmed Molecular characterization of the first leptospires isolated from goats in Brazil
title_sort Molecular characterization of the first leptospires isolated from goats in Brazil
author Lilenbaum,Walter
author_facet Lilenbaum,Walter
Kremer,Frederico
Ristow,Paula
Dellagostin,Odir
Bourhy,Pascale
Hartskeerl,Rudy
Vasconcellos,Silvio
author_role author
author2 Kremer,Frederico
Ristow,Paula
Dellagostin,Odir
Bourhy,Pascale
Hartskeerl,Rudy
Vasconcellos,Silvio
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Lilenbaum,Walter
Kremer,Frederico
Ristow,Paula
Dellagostin,Odir
Bourhy,Pascale
Hartskeerl,Rudy
Vasconcellos,Silvio
dc.subject.por.fl_str_mv Leptospira
serogrouping
sequencing
PFGE
rrs
topic Leptospira
serogrouping
sequencing
PFGE
rrs
description Two Leptospira sp. isolates were obtained by the first time from goats in Brazil and characterized by sequencing rrs, rpoB and secY genes, PFGE and typing with monoclonal antibodies. Both isolates are identical and belong to Leptospira santarosai. Analysis of the rrs and the rpoB genes sequences revealed 100% identity between the goat isolates and the Bananal reference strain. When secY sequences of the two isolates were compared to each other, it was observed that they had identical sequences. However, when compared to that of the Bananal reference strain, there were 15 mismatches along the 549 bp secY sequence. In conclusion, molecular methods are increasingly useful for the characterization of leptospires and allowed to identify those isolates of caprine origin as closely related but not identical to serovar Bananal, and constitute a new type named Carioca.
publishDate 2014
dc.date.none.fl_str_mv 2014-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400050
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000400050
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.4 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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