LTB-R1: an alternative to swine mycoplasmal pneumoniae control

Detalhes bibliográficos
Autor(a) principal: Conceição,Fabrício Rochedo
Data de Publicação: 2003
Outros Autores: Michelon,André, Michelon,Marcelo, Cerqueira,Gustavo Maia de, Dellagostin,Odir Antonio
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500027
Resumo: Mycoplasmal pneumoniae is the main respiratory disease in swine. The most efficient way to control it is through the use of vaccines (bacterins), whose production cost is high. The objective of this work was to develop a new alternative for controlling Swine Mycoplasmal Pneumoniae, based on a recombinant subunit vaccine containing the R1 region of P97 adhesin of Mycoplasma hyopneumoniae fused to the B subunit of the heat-labile enterotoxin of Escherichia coli (rLTB-R1). In this work we report the amplification of the genes, genetic fusion between LTB and R1 coding sequences, cloning, construction of the expression vector, as well as expression and purification of rLTB-R1 in E. coli.
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spelling LTB-R1: an alternative to swine mycoplasmal pneumoniae controlswine mycoplasmal pneumoniaeP97LTBMycoplasmal pneumoniae is the main respiratory disease in swine. The most efficient way to control it is through the use of vaccines (bacterins), whose production cost is high. The objective of this work was to develop a new alternative for controlling Swine Mycoplasmal Pneumoniae, based on a recombinant subunit vaccine containing the R1 region of P97 adhesin of Mycoplasma hyopneumoniae fused to the B subunit of the heat-labile enterotoxin of Escherichia coli (rLTB-R1). In this work we report the amplification of the genes, genetic fusion between LTB and R1 coding sequences, cloning, construction of the expression vector, as well as expression and purification of rLTB-R1 in E. coli.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500027Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500027info:eu-repo/semantics/openAccessConceição,Fabrício RochedoMichelon,AndréMichelon,MarceloCerqueira,Gustavo Maia deDellagostin,Odir Antonioeng2004-11-29T00:00:00Zoai:scielo:S1517-83822003000500027Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv LTB-R1: an alternative to swine mycoplasmal pneumoniae control
title LTB-R1: an alternative to swine mycoplasmal pneumoniae control
spellingShingle LTB-R1: an alternative to swine mycoplasmal pneumoniae control
Conceição,Fabrício Rochedo
swine mycoplasmal pneumoniae
P97
LTB
title_short LTB-R1: an alternative to swine mycoplasmal pneumoniae control
title_full LTB-R1: an alternative to swine mycoplasmal pneumoniae control
title_fullStr LTB-R1: an alternative to swine mycoplasmal pneumoniae control
title_full_unstemmed LTB-R1: an alternative to swine mycoplasmal pneumoniae control
title_sort LTB-R1: an alternative to swine mycoplasmal pneumoniae control
author Conceição,Fabrício Rochedo
author_facet Conceição,Fabrício Rochedo
Michelon,André
Michelon,Marcelo
Cerqueira,Gustavo Maia de
Dellagostin,Odir Antonio
author_role author
author2 Michelon,André
Michelon,Marcelo
Cerqueira,Gustavo Maia de
Dellagostin,Odir Antonio
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Conceição,Fabrício Rochedo
Michelon,André
Michelon,Marcelo
Cerqueira,Gustavo Maia de
Dellagostin,Odir Antonio
dc.subject.por.fl_str_mv swine mycoplasmal pneumoniae
P97
LTB
topic swine mycoplasmal pneumoniae
P97
LTB
description Mycoplasmal pneumoniae is the main respiratory disease in swine. The most efficient way to control it is through the use of vaccines (bacterins), whose production cost is high. The objective of this work was to develop a new alternative for controlling Swine Mycoplasmal Pneumoniae, based on a recombinant subunit vaccine containing the R1 region of P97 adhesin of Mycoplasma hyopneumoniae fused to the B subunit of the heat-labile enterotoxin of Escherichia coli (rLTB-R1). In this work we report the amplification of the genes, genetic fusion between LTB and R1 coding sequences, cloning, construction of the expression vector, as well as expression and purification of rLTB-R1 in E. coli.
publishDate 2003
dc.date.none.fl_str_mv 2003-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500027
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500027
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000500027
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 suppl.1 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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