Isolation and characterization of novel thermophilic lipase-secreting bacteria

• Autor(a) principal: Rabbani,Mohammed
• Data de Publicação: 2013
• Outros Autores: Bagherinejad,Mohammad Reza, Sadeghi,Hamid MirMohammad, Shariat,Ziaedin Samsam, Etemadifar,Zahra, Moazen,Fatemeh, Rahbari,Manizheh, Mafakher,Ladan, Zaghian,Saeideh
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400013
• topic: extracellular lipase; pH-stat method; thermophilic lipase; fermentation

The purpose of the present study was to screen and identify the lipase-producing microorganisms from various regions of Iran. Samples collected from hot spring, Persian Gulf, desert area and oil-contaminated soil, were analyzed for thermophilic extracellular-lipase producing organisms. Six strains with high activity on rhodamine B plates were selected for chemical identification and further study. Among these isolated bacteria, four strains show higher activity in pH-Stat method at 55 °C. These strains were identified by PCR amplification of 16s rRNA genes using universal primers. Fermentation increased the activity up to 50%. The growth medium, designed for lipase production, increased the activity up to 4.55 folds. The crude supernatant of ZR-5 after fermentation and separation the cells, was lyophilized and the activity was measured. Total activity of this strain was 12 kU/g that shows its potential for industrial uses. Further study is required for purification of enzyme and calculation its specific activity. Immobilization is another approach should be considered.