Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68
Autor(a) principal: | |
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Data de Publicação: | 2008 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027 |
Resumo: | An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by SDS and EDTA by 38% and 34%, respectively. |
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Brazilian Journal of Microbiology |
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Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68Bacillus sp.α-amylasealkaliphilicthermostableenzymeAn alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by SDS and EDTA by 38% and 34%, respectively.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300027info:eu-repo/semantics/openAccessAygan,AshabilArikan,BurhanKorkmaz,HaticeDinçer,SadikÇolak,Ömereng2008-10-03T00:00:00Zoai:scielo:S1517-83822008000300027Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
title |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
spellingShingle |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 Aygan,Ashabil Bacillus sp. α-amylase alkaliphilic thermostable enzyme |
title_short |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
title_full |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
title_fullStr |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
title_full_unstemmed |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
title_sort |
Highly thermostable and alkaline α-amylase from a halotolerant-alkaliphilic Bacillus sp. AB68 |
author |
Aygan,Ashabil |
author_facet |
Aygan,Ashabil Arikan,Burhan Korkmaz,Hatice Dinçer,Sadik Çolak,Ömer |
author_role |
author |
author2 |
Arikan,Burhan Korkmaz,Hatice Dinçer,Sadik Çolak,Ömer |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Aygan,Ashabil Arikan,Burhan Korkmaz,Hatice Dinçer,Sadik Çolak,Ömer |
dc.subject.por.fl_str_mv |
Bacillus sp. α-amylase alkaliphilic thermostable enzyme |
topic |
Bacillus sp. α-amylase alkaliphilic thermostable enzyme |
description |
An alkaliphilic and highly thermostable α-amylase producing Bacillus sp. was isolated from Van soda lake. Enzyme synthesis occurred at temperatures between 25ºC and 40ºC. Analysis of the enzyme by SDS-PAGE revealed a single band which was estimated to be 66 kDa. The enzyme was active in a broad temperature range, between 20ºC and 90ºC, with an optimum at 50ºC; and maximum activity was at pH 10.5. The enzyme was almost completely stable up to 80ºC with a remaining activity over 90% after 30 min pre-incubation. Thermostability was not increased in the presence of Ca2+. An average of 75% and 60ºC of remaining activity was observed when the enzyme was incubated between pH 5 and 9 for 1 h and for 2 h, respectively. The activity of the enzyme was inhibited by SDS and EDTA by 38% and 34%, respectively. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300027 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000300027 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.3 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201872531456 |