Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028 |
Resumo: | Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%) and L. gasseri (20.6%) were the most frequent species. However, using the biochemical technique, the most frequent species were: L. acidophilus (34.8%), L. crispatus (27.2%) and L. fermentum (13%). Although L. acidophilus was the most frequent specie found by biochemical tests, no strain of this microorganism was detected by PCR. Agreement between the methods was low for identification of all the most common species. Although rates of L. crispatus detected were similar using both methods (32.6% and 27.2%), agreement between them was relatively low (kappa = 0.52). Conclusions: Our results confirmed the limitation of the biochemical method and the applicability of a previously published molecular method (Multiplex PCR) for the identification of lactobacilli in the vaginal tract, focusing on further necessity of its improvement for also targeting L. vaginalis and L. iners. |
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Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolatescarbohydrate fermentation testmultiplex PCRidentificationvaginal lactobacillivaginal ecosystemIdentification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%) and L. gasseri (20.6%) were the most frequent species. However, using the biochemical technique, the most frequent species were: L. acidophilus (34.8%), L. crispatus (27.2%) and L. fermentum (13%). Although L. acidophilus was the most frequent specie found by biochemical tests, no strain of this microorganism was detected by PCR. Agreement between the methods was low for identification of all the most common species. Although rates of L. crispatus detected were similar using both methods (32.6% and 27.2%), agreement between them was relatively low (kappa = 0.52). Conclusions: Our results confirmed the limitation of the biochemical method and the applicability of a previously published molecular method (Multiplex PCR) for the identification of lactobacilli in the vaginal tract, focusing on further necessity of its improvement for also targeting L. vaginalis and L. iners.Sociedade Brasileira de Microbiologia2011-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028Brazilian Journal of Microbiology v.42 n.1 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000100028info:eu-repo/semantics/openAccessBrolazo,Eliane MeloLeite,Domingos SilvaTiba,Monique RibeiroVillarroel,MarinaMarconi,CamilaSimoes,Jose Antonioeng2011-01-10T00:00:00Zoai:scielo:S1517-83822011000100028Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-01-10T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
title |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
spellingShingle |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates Brolazo,Eliane Melo carbohydrate fermentation test multiplex PCR identification vaginal lactobacilli vaginal ecosystem |
title_short |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
title_full |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
title_fullStr |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
title_full_unstemmed |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
title_sort |
Correlation between API 50 CH and multiplex polymerase chain reaction for the identification of vaginal lactobacilli in isolates |
author |
Brolazo,Eliane Melo |
author_facet |
Brolazo,Eliane Melo Leite,Domingos Silva Tiba,Monique Ribeiro Villarroel,Marina Marconi,Camila Simoes,Jose Antonio |
author_role |
author |
author2 |
Leite,Domingos Silva Tiba,Monique Ribeiro Villarroel,Marina Marconi,Camila Simoes,Jose Antonio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Brolazo,Eliane Melo Leite,Domingos Silva Tiba,Monique Ribeiro Villarroel,Marina Marconi,Camila Simoes,Jose Antonio |
dc.subject.por.fl_str_mv |
carbohydrate fermentation test multiplex PCR identification vaginal lactobacilli vaginal ecosystem |
topic |
carbohydrate fermentation test multiplex PCR identification vaginal lactobacilli vaginal ecosystem |
description |
Identification of Lactobacillus sp. strains by phenotypic methods may lead to doubtful results possibly interfering in the reliability of the epidemiological and probiotics studies. Therefore this study aimed to determine the best methodology for the identification of the large diversity of lactobacilli species found in the vagina by comparing two techniques, one based on their biochemical profile and other employing molecular biology. A carbohydrate fermentation test (API 50 CH) was compared with multiplex polymerase chain reaction (PCR) for the identification of species of vaginal lactobacilli from 135 healthy women. The kappa index was used to evaluate agreement between the methods. Using the molecular technique, L. crispatus (32.6%), L. jensenii (25%) and L. gasseri (20.6%) were the most frequent species. However, using the biochemical technique, the most frequent species were: L. acidophilus (34.8%), L. crispatus (27.2%) and L. fermentum (13%). Although L. acidophilus was the most frequent specie found by biochemical tests, no strain of this microorganism was detected by PCR. Agreement between the methods was low for identification of all the most common species. Although rates of L. crispatus detected were similar using both methods (32.6% and 27.2%), agreement between them was relatively low (kappa = 0.52). Conclusions: Our results confirmed the limitation of the biochemical method and the applicability of a previously published molecular method (Multiplex PCR) for the identification of lactobacilli in the vaginal tract, focusing on further necessity of its improvement for also targeting L. vaginalis and L. iners. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000100028 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000100028 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.1 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203483144192 |