Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity
Autor(a) principal: | |
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Data de Publicação: | 2010 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011 |
Resumo: | Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA assays. Twelve drugs were evaluated for their antimicrobial effects on culture RGE-treated cells using the disk diffusion method. Penicillin resistant strains became sensitive to the drug after RGE treatment. Furthermore, enterotoxin production by RGE-treated S. aureus was evaluated using a standardized ELISA method. Although staphylococcal LSA 88 bovine strain cells remained viable after exposure to the extract, enterotoxin production was precluded in 20% after RGE treatment. Significant interference in staphylococci cell density, drug sensitivity and enterotoxin secretion was observed after treatment. The study highlights the necessity to find new methods of disease prevention and new antibiotic therapies against staphylococcal infections. |
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Brazilian Journal of Microbiology |
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Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activityRauvolfia grandifloraStaphylococcus aureusenterotoxinantimicrobial activityStaphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA assays. Twelve drugs were evaluated for their antimicrobial effects on culture RGE-treated cells using the disk diffusion method. Penicillin resistant strains became sensitive to the drug after RGE treatment. Furthermore, enterotoxin production by RGE-treated S. aureus was evaluated using a standardized ELISA method. Although staphylococcal LSA 88 bovine strain cells remained viable after exposure to the extract, enterotoxin production was precluded in 20% after RGE treatment. Significant interference in staphylococci cell density, drug sensitivity and enterotoxin secretion was observed after treatment. The study highlights the necessity to find new methods of disease prevention and new antibiotic therapies against staphylococcal infections.Sociedade Brasileira de Microbiologia2010-10-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011Brazilian Journal of Microbiology v.41 n.3 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000300011info:eu-repo/semantics/openAccessCarlos,Lanamar de AlmeidaAmaral,Kenas Aguiar da SilvaVieira,Ivo José CurcinoMathias,LedaBraz-Filho,RaimundoSamarão,Solange SilvaVieira-da-Motta,Olneyeng2010-06-02T00:00:00Zoai:scielo:S1517-83822010000300011Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-06-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
title |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
spellingShingle |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity Carlos,Lanamar de Almeida Rauvolfia grandiflora Staphylococcus aureus enterotoxin antimicrobial activity |
title_short |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
title_full |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
title_fullStr |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
title_full_unstemmed |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
title_sort |
Rauvolfia grandiflora (Apocynaceae) extract interferes with staphylococcal density, enterotoxin production and antimicrobial activity |
author |
Carlos,Lanamar de Almeida |
author_facet |
Carlos,Lanamar de Almeida Amaral,Kenas Aguiar da Silva Vieira,Ivo José Curcino Mathias,Leda Braz-Filho,Raimundo Samarão,Solange Silva Vieira-da-Motta,Olney |
author_role |
author |
author2 |
Amaral,Kenas Aguiar da Silva Vieira,Ivo José Curcino Mathias,Leda Braz-Filho,Raimundo Samarão,Solange Silva Vieira-da-Motta,Olney |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Carlos,Lanamar de Almeida Amaral,Kenas Aguiar da Silva Vieira,Ivo José Curcino Mathias,Leda Braz-Filho,Raimundo Samarão,Solange Silva Vieira-da-Motta,Olney |
dc.subject.por.fl_str_mv |
Rauvolfia grandiflora Staphylococcus aureus enterotoxin antimicrobial activity |
topic |
Rauvolfia grandiflora Staphylococcus aureus enterotoxin antimicrobial activity |
description |
Staphylococci bacteria are involved in many human and animal infections and development of alternative antimicrobial drugs against pathogenic bacteria is of great interest to the pharmaceutical industry. This study investigated the in vitro effect of Rauvolfia grandiflora methanol extract (root bark fraction) (RGE) on the density of ATCC strains of Staphylococcus aureus and Staphylococcus epidermidis, and a clinical enterotoxin-producer, S. aureus bovine strain. The alkaloid, isoreserpiline, obtained from dichloromethane extract of R. grandiflora was ineffective against the strains tested. After incubation of staphylococci strains in the presence of 1.2 mg.mL-1 RGE, a significant inhibition of cell growth was observed using both spectrophotometry and ELISA assays. Twelve drugs were evaluated for their antimicrobial effects on culture RGE-treated cells using the disk diffusion method. Penicillin resistant strains became sensitive to the drug after RGE treatment. Furthermore, enterotoxin production by RGE-treated S. aureus was evaluated using a standardized ELISA method. Although staphylococcal LSA 88 bovine strain cells remained viable after exposure to the extract, enterotoxin production was precluded in 20% after RGE treatment. Significant interference in staphylococci cell density, drug sensitivity and enterotoxin secretion was observed after treatment. The study highlights the necessity to find new methods of disease prevention and new antibiotic therapies against staphylococcal infections. |
publishDate |
2010 |
dc.date.none.fl_str_mv |
2010-10-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000300011 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822010000300011 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.41 n.3 2010 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203046936576 |