Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100210 |
Resumo: | Abstract Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida. |
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Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swinePasteurellosisVirulence factorsMultiplex-PCRAntimicrobial susceptibilityAbstract Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida.Sociedade Brasileira de Microbiologia2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100210Brazilian Journal of Microbiology v.47 n.1 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2015.11.014info:eu-repo/semantics/openAccessFurian,Thales QuediBorges,Karen ApellanisLaviniki,VanessaRocha,Silvio Luis da SilveiraAlmeida,Camila Neves deNascimento,Vladimir Pinheiro doSalle,Carlos Tadeu PippiMoraes,Hamilton Luiz de Souzaeng2016-03-01T00:00:00Zoai:scielo:S1517-83822016000100210Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-03-01T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
title |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
spellingShingle |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine Furian,Thales Quedi Pasteurellosis Virulence factors Multiplex-PCR Antimicrobial susceptibility |
title_short |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
title_full |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
title_fullStr |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
title_full_unstemmed |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
title_sort |
Virulence genes and antimicrobial resistance of Pasteurella multocida isolated from poultry and swine |
author |
Furian,Thales Quedi |
author_facet |
Furian,Thales Quedi Borges,Karen Apellanis Laviniki,Vanessa Rocha,Silvio Luis da Silveira Almeida,Camila Neves de Nascimento,Vladimir Pinheiro do Salle,Carlos Tadeu Pippi Moraes,Hamilton Luiz de Souza |
author_role |
author |
author2 |
Borges,Karen Apellanis Laviniki,Vanessa Rocha,Silvio Luis da Silveira Almeida,Camila Neves de Nascimento,Vladimir Pinheiro do Salle,Carlos Tadeu Pippi Moraes,Hamilton Luiz de Souza |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Furian,Thales Quedi Borges,Karen Apellanis Laviniki,Vanessa Rocha,Silvio Luis da Silveira Almeida,Camila Neves de Nascimento,Vladimir Pinheiro do Salle,Carlos Tadeu Pippi Moraes,Hamilton Luiz de Souza |
dc.subject.por.fl_str_mv |
Pasteurellosis Virulence factors Multiplex-PCR Antimicrobial susceptibility |
topic |
Pasteurellosis Virulence factors Multiplex-PCR Antimicrobial susceptibility |
description |
Abstract Pasteurella multocida causes atrophic rhinitis in swine and fowl cholera in birds, and is a secondary agent in respiratory syndromes. Pathogenesis and virulence factors involved are still poorly understood. The aim of this study was to detect 22 virulence-associated genes by PCR, including capsular serogroups A, B and D genes and to evaluate the antimicrobial susceptibility of P. multocida strains from poultry and swine. ompH, oma87, plpB, psl, exbD-tonB, fur, hgbA, nanB, sodA, sodC, ptfA were detected in more than 90% of the strains of both hosts. 91% and 92% of avian and swine strains, respectively, were classified in serogroup A. toxA and hsf-1 showed a significant association to serogroup D; pmHAS and pfhA to serogroup A. Gentamicin and amoxicillin were the most effective drugs with susceptibility higher than 97%; however, 76.79% of poultry strains and 85% of swine strains were resistant to sulphonamides. Furthermore, 19.64% and 36.58% of avian and swine strains, respectively, were multi-resistant. Virulence genes studied were not specific to a host and may be the result of horizontal transmission throughout evolution. High multidrug resistance demonstrates the need for responsible use of antimicrobials in animals intended for human consumption, in addition to antimicrobial susceptibility testing to P. multocida. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-03-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100210 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100210 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2015.11.014 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.1 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122208308690944 |