Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300040 |
Resumo: | In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract. |
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Brazilian Journal of Microbiology |
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Identification and adhesion profile of Lactobacillus spp. strains isolated from poultryLactobacillusidentificationintestinal adhesionIn the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract.Sociedade Brasileira de Microbiologia2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300040Brazilian Journal of Microbiology v.45 n.3 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000300040info:eu-repo/semantics/openAccessRocha,Ticiana SilvaBaptista,Ana Angelita SampaioDonato,Tais CremascoMilbradt,Elisane LenitaOkamoto,Adriano SakaiAndreatti Filho,Raphael Lucioeng2014-11-04T00:00:00Zoai:scielo:S1517-83822014000300040Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-11-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
title |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
spellingShingle |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry Rocha,Ticiana Silva Lactobacillus identification intestinal adhesion |
title_short |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
title_full |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
title_fullStr |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
title_full_unstemmed |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
title_sort |
Identification and adhesion profile of Lactobacillus spp. strains isolated from poultry |
author |
Rocha,Ticiana Silva |
author_facet |
Rocha,Ticiana Silva Baptista,Ana Angelita Sampaio Donato,Tais Cremasco Milbradt,Elisane Lenita Okamoto,Adriano Sakai Andreatti Filho,Raphael Lucio |
author_role |
author |
author2 |
Baptista,Ana Angelita Sampaio Donato,Tais Cremasco Milbradt,Elisane Lenita Okamoto,Adriano Sakai Andreatti Filho,Raphael Lucio |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Rocha,Ticiana Silva Baptista,Ana Angelita Sampaio Donato,Tais Cremasco Milbradt,Elisane Lenita Okamoto,Adriano Sakai Andreatti Filho,Raphael Lucio |
dc.subject.por.fl_str_mv |
Lactobacillus identification intestinal adhesion |
topic |
Lactobacillus identification intestinal adhesion |
description |
In the aviculture industry, the use of Lactobacillus spp. as a probiotic has been shown to be frequent and satisfactory, both in improving bird production indexes and in protecting intestine against colonization by pathogenic bacteria. Adhesion is an important characteristic in selecting Lactobacillus probiotic strains since it impedes its immediate elimination to enable its beneficial action in the host. This study aimed to isolate, identify and characterize the in vitro and in vivo adhesion of Lactobacillus strains isolated from birds. The Lactobacillus spp. was identified by PCR and sequencing and the strains and its adhesion evaluated in vitro via BMM cell matrix and in vivo by inoculation in one-day-old birds. Duodenum, jejunum, ileum and cecum were collected one, four, 12 and 24 h after inoculation. The findings demonstrate greater adhesion of strains in the cecum and an important correlation between in vitro and in vivo results. It was concluded that BMM utilization represents an important technique for triage of Lactobacillus for subsequent in vivo evaluation, which was shown to be efficient in identifying bacterial adhesion to the enteric tract. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300040 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822014000300040 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.45 n.3 2014 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122206635163648 |