KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501 |
Resumo: | The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009. |
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Brazilian Journal of Microbiology |
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KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of BrazilKlebsiella pneumoniaecarbapenemsdrug-resistant bacteriaThe emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.Sociedade Brasileira de Microbiologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501Brazilian Journal of Microbiology v.46 n.2 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246246220140174info:eu-repo/semantics/openAccessBiberg,Camila ArgueloRodrigues,Ana Claudia SouzaCarmo,Sidiane Ferreira doChaves,Claudia Elizabeth VolpeGales,Ana CristinaChang,Marilene Rodrigueseng2015-11-27T00:00:00Zoai:scielo:S1517-83822015000200501Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-11-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
title |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
spellingShingle |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil Biberg,Camila Arguelo Klebsiella pneumoniae carbapenems drug-resistant bacteria |
title_short |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
title_full |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
title_fullStr |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
title_full_unstemmed |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
title_sort |
KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil |
author |
Biberg,Camila Arguelo |
author_facet |
Biberg,Camila Arguelo Rodrigues,Ana Claudia Souza Carmo,Sidiane Ferreira do Chaves,Claudia Elizabeth Volpe Gales,Ana Cristina Chang,Marilene Rodrigues |
author_role |
author |
author2 |
Rodrigues,Ana Claudia Souza Carmo,Sidiane Ferreira do Chaves,Claudia Elizabeth Volpe Gales,Ana Cristina Chang,Marilene Rodrigues |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Biberg,Camila Arguelo Rodrigues,Ana Claudia Souza Carmo,Sidiane Ferreira do Chaves,Claudia Elizabeth Volpe Gales,Ana Cristina Chang,Marilene Rodrigues |
dc.subject.por.fl_str_mv |
Klebsiella pneumoniae carbapenems drug-resistant bacteria |
topic |
Klebsiella pneumoniae carbapenems drug-resistant bacteria |
description |
The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-06-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-838246246220140174 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.46 n.2 2015 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122207463538688 |