KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil

Detalhes bibliográficos
Autor(a) principal: Biberg,Camila Arguelo
Data de Publicação: 2015
Outros Autores: Rodrigues,Ana Claudia Souza, Carmo,Sidiane Ferreira do, Chaves,Claudia Elizabeth Volpe, Gales,Ana Cristina, Chang,Marilene Rodrigues
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501
Resumo: The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.
id SBM-1_4ec7ee48f7f6e0b53a314dea9e46e90a
oai_identifier_str oai:scielo:S1517-83822015000200501
network_acronym_str SBM-1
network_name_str Brazilian Journal of Microbiology
repository_id_str
spelling KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of BrazilKlebsiella pneumoniaecarbapenemsdrug-resistant bacteriaThe emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.Sociedade Brasileira de Microbiologia2015-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501Brazilian Journal of Microbiology v.46 n.2 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246246220140174info:eu-repo/semantics/openAccessBiberg,Camila ArgueloRodrigues,Ana Claudia SouzaCarmo,Sidiane Ferreira doChaves,Claudia Elizabeth VolpeGales,Ana CristinaChang,Marilene Rodrigueseng2015-11-27T00:00:00Zoai:scielo:S1517-83822015000200501Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-11-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
title KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
spellingShingle KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
Biberg,Camila Arguelo
Klebsiella pneumoniae
carbapenems
drug-resistant bacteria
title_short KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
title_full KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
title_fullStr KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
title_full_unstemmed KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
title_sort KPC-2-producing Klebsiella pneumoniae in a hospital in the Midwest region of Brazil
author Biberg,Camila Arguelo
author_facet Biberg,Camila Arguelo
Rodrigues,Ana Claudia Souza
Carmo,Sidiane Ferreira do
Chaves,Claudia Elizabeth Volpe
Gales,Ana Cristina
Chang,Marilene Rodrigues
author_role author
author2 Rodrigues,Ana Claudia Souza
Carmo,Sidiane Ferreira do
Chaves,Claudia Elizabeth Volpe
Gales,Ana Cristina
Chang,Marilene Rodrigues
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Biberg,Camila Arguelo
Rodrigues,Ana Claudia Souza
Carmo,Sidiane Ferreira do
Chaves,Claudia Elizabeth Volpe
Gales,Ana Cristina
Chang,Marilene Rodrigues
dc.subject.por.fl_str_mv Klebsiella pneumoniae
carbapenems
drug-resistant bacteria
topic Klebsiella pneumoniae
carbapenems
drug-resistant bacteria
description The emergence of β-lactamase-producing Enterobacteriaceae in the last few decades has become major challenge faced by hospitals. In this study, isolates of Klebsiella pneumoniae carbapenemase-2 (KPC-2)-producing K. pneumoniae from a tertiary hospital in Mato Grosso do Sul, Brazil, were characterized. Bacterial identification was performed by matrix-assisted laser desorption/ionization time-of-flight (MALDI-TOF; Bruker Daltonics, Germany) mass spectrometry. The minimum inhibitory concentrations of carbapenems were determined using the agar dilution method as recommended by the Clinical Laboratory Standards Institute guidelines. Carbapenemase production was detected using the modified Hodge test (MHT) and polymerase chain reaction (PCR), followed by DNA sequencing. Of 360 (12.2%) K. pneumoniae isolates obtained between May 2009 and May 2010, 44 (12.2%) were carbapenem nonsusceptible. Of these 44 isolates, thirty-six K. pneumoniae isolates that were positive by MHT and PCR carried the blaKPC-2 gene. Thus, KPC-2producing Klebsiella pneumoniae has been present in a Brazilian hospital located in the Midwest region since at least 2009.
publishDate 2015
dc.date.none.fl_str_mv 2015-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000200501
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246246220140174
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.2 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
_version_ 1752122207463538688