Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa

Detalhes bibliográficos
Autor(a) principal: Lima,Ilana Schneider
Data de Publicação: 2014
Outros Autores: Baumeier,Nicole Carmo, Rosa,Rosimeire Takaki, Campelo,Patrícia Maria Stuelp, Rosa,Edvaldo Antonio Ribeiro
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300029
Resumo: This study evaluated the impact of different concentrations of glyphosate (Rondup®) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC®15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their OD560nm every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand, the herbicide favors a slight growth of biofilms in a concentration-dependent manner up to 84.5 ppm (p &gt; 0.05), and more pronounced over 169 ppm. Anaerobic biofilms have their growth more readily favored (p < 0.05), regardless of concentration. In a concentration-dependent manner, glyphosate interferes with the growth ability of P. aeruginosa ATCC®15442.
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spelling Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosaPseudomonas aeruginosaglyphosategrowthbiofilmThis study evaluated the impact of different concentrations of glyphosate (Rondup®) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC®15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their OD560nm every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand, the herbicide favors a slight growth of biofilms in a concentration-dependent manner up to 84.5 ppm (p &gt; 0.05), and more pronounced over 169 ppm. Anaerobic biofilms have their growth more readily favored (p < 0.05), regardless of concentration. In a concentration-dependent manner, glyphosate interferes with the growth ability of P. aeruginosa ATCC®15442.Sociedade Brasileira de Microbiologia2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300029Brazilian Journal of Microbiology v.45 n.3 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000300029info:eu-repo/semantics/openAccessLima,Ilana SchneiderBaumeier,Nicole CarmoRosa,Rosimeire TakakiCampelo,Patrícia Maria StuelpRosa,Edvaldo Antonio Ribeiroeng2014-11-04T00:00:00Zoai:scielo:S1517-83822014000300029Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-11-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
spellingShingle Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
Lima,Ilana Schneider
Pseudomonas aeruginosa
glyphosate
growth
biofilm
title_short Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_full Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_fullStr Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_full_unstemmed Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
title_sort Influence of glyphosate in planktonic and biofilm growth of Pseudomonas aeruginosa
author Lima,Ilana Schneider
author_facet Lima,Ilana Schneider
Baumeier,Nicole Carmo
Rosa,Rosimeire Takaki
Campelo,Patrícia Maria Stuelp
Rosa,Edvaldo Antonio Ribeiro
author_role author
author2 Baumeier,Nicole Carmo
Rosa,Rosimeire Takaki
Campelo,Patrícia Maria Stuelp
Rosa,Edvaldo Antonio Ribeiro
author2_role author
author
author
author
dc.contributor.author.fl_str_mv Lima,Ilana Schneider
Baumeier,Nicole Carmo
Rosa,Rosimeire Takaki
Campelo,Patrícia Maria Stuelp
Rosa,Edvaldo Antonio Ribeiro
dc.subject.por.fl_str_mv Pseudomonas aeruginosa
glyphosate
growth
biofilm
topic Pseudomonas aeruginosa
glyphosate
growth
biofilm
description This study evaluated the impact of different concentrations of glyphosate (Rondup®) on planktonic and biofilm growth of P. aeruginosa. Aerobic and anaerobic cultures of P. aeruginosa ATCC®15442 inoculated in MHB + glyphosate (0.845 ppm, 1.690 ppm, 8.45 ppm, 16.90 ppm, 84.50 ppm, 169 ppm, 845 ppm, and 1690 ppm) and cultured in normoxia and anoxia, following their OD560nm every hour for 24 h. Biofilms of adapted cells were formed in the presence of glyphosate (0.845 to 1690 ppm) in normoxia and anoxia for 36 h. Glyphosate at concentrations higher than 84.5 ppm reduces the cell density of planktonic aerobic cultures (p < 0.05). However, these same concentrations favor the planktonic anaerobic growth (p < 0.05). On the other hand, the herbicide favors a slight growth of biofilms in a concentration-dependent manner up to 84.5 ppm (p &gt; 0.05), and more pronounced over 169 ppm. Anaerobic biofilms have their growth more readily favored (p < 0.05), regardless of concentration. In a concentration-dependent manner, glyphosate interferes with the growth ability of P. aeruginosa ATCC®15442.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300029
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300029
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000300029
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.3 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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