Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400007 |
Resumo: | The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment time. Such an approach allowed us to identify an optimal range of the independent variables within which the β-galactosidase activity was optimized. A maximum permeabilization of 2,816 mmol L-1 oNP min-1 g-1 was obtained by treating cells with 75.0% v/v of ethanol at 20.0 °C for 15.0 min. The proposed methodology resulted to be effective and suited for K. lactis cells permeabilization at a lab-scale and promises to be of possible interest for future applications mainly in the food industry. |
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Brazilian Journal of Microbiology |
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Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodologyyeastcheese wheypermeabilizationethanolβ-galactosidaseThe aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment time. Such an approach allowed us to identify an optimal range of the independent variables within which the β-galactosidase activity was optimized. A maximum permeabilization of 2,816 mmol L-1 oNP min-1 g-1 was obtained by treating cells with 75.0% v/v of ethanol at 20.0 °C for 15.0 min. The proposed methodology resulted to be effective and suited for K. lactis cells permeabilization at a lab-scale and promises to be of possible interest for future applications mainly in the food industry.Sociedade Brasileira de Microbiologia2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400007Brazilian Journal of Microbiology v.44 n.4 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013000400007info:eu-repo/semantics/openAccessFaria,Janaína T. deRocha,Pollyana F.Converti,AttilioPassos,Flávia M.L.Minim,Luis A.Sampaio,Fábio C.eng2014-03-27T00:00:00Zoai:scielo:S1517-83822013000400007Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-03-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
title |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
spellingShingle |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology Faria,Janaína T. de yeast cheese whey permeabilization ethanol β-galactosidase |
title_short |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
title_full |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
title_fullStr |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
title_full_unstemmed |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
title_sort |
Statistical investigation of Kluyveromyces lactis cells permeabilization with ethanol by response surface methodology |
author |
Faria,Janaína T. de |
author_facet |
Faria,Janaína T. de Rocha,Pollyana F. Converti,Attilio Passos,Flávia M.L. Minim,Luis A. Sampaio,Fábio C. |
author_role |
author |
author2 |
Rocha,Pollyana F. Converti,Attilio Passos,Flávia M.L. Minim,Luis A. Sampaio,Fábio C. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Faria,Janaína T. de Rocha,Pollyana F. Converti,Attilio Passos,Flávia M.L. Minim,Luis A. Sampaio,Fábio C. |
dc.subject.por.fl_str_mv |
yeast cheese whey permeabilization ethanol β-galactosidase |
topic |
yeast cheese whey permeabilization ethanol β-galactosidase |
description |
The aim of our study was to select the optimal operating conditions to permeabilize Kluyveromyces lactis cells using ethanol as a solvent as an alternative to cell disruption and extraction. Cell permeabilization was carried out by a non-mechanical method consisting of chemical treatment with ethanol, and the results were expressed as β-galactosidase activity. Experiments were conducted under different conditions of ethanol concentration, treatment time and temperature according to a central composite rotatable design (CCRD), and the collected results were then worked out by response surface methodology (RSM). Cell permeabilization was improved by an increase in ethanol concentration and simultaneous decreases in the incubation temperature and treatment time. Such an approach allowed us to identify an optimal range of the independent variables within which the β-galactosidase activity was optimized. A maximum permeabilization of 2,816 mmol L-1 oNP min-1 g-1 was obtained by treating cells with 75.0% v/v of ethanol at 20.0 °C for 15.0 min. The proposed methodology resulted to be effective and suited for K. lactis cells permeabilization at a lab-scale and promises to be of possible interest for future applications mainly in the food industry. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400007 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400007 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822013000400007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.4 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122205636919296 |