Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions

Detalhes bibliográficos
Autor(a) principal: Silva,Fábio Sérgio B.
Data de Publicação: 2005
Outros Autores: Yano-Melo,Adriana M., Brandão,Joana Angélica C., Maia,Leonor C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400004
Resumo: The production of inoculum is one of the hindrances in the large scale application of arbuscular mycorrhizal fungi (AMF). The objective of this work was to evaluate the effect of nutrient solutions with or without Tris-HCl buffer, on sporulation of AMF. The experiment was carried out in a greenhouse, using a substrate with sand and vermiculite (1:1 v/v). Fifty spores of Gigaspora margarita, Scutellospora heterogama, and Glomus etunicatum were inoculated in Sorghum vulgare (sorghum) or Panicum miliaceum (fodder millet). The substrate received the following nutrient solutions: Hoagland with 3 muM P (S1); Long Ashton II with 15.9 muM P (S2) and Hoagland with 20 muM P (S3), with or without 50 mM of Tris-HCl buffer (pH 6.5); the control treatment, consisting of a soil + sand + vermiculite (2:1:1 v/v) substrate was irrigated with deionized water. Ten weeks after the beginning of the experiment sporulation did not differ in treatments with sorghum. Panicum miliaceum promoted higher sporulation of the AMF than sorghum, and differences among treatments with nutrient solutions were observed. Production of spores of G. margarita and S. heterogama increased significantly after addition of buffer in S1 and S2, while that of G. etunicatum was improved when the substrate was irrigated with S1 + buffer and S3 solutions. Solution S1 + buffer benefited sporulation of the three fungi. However, as observed, each AMF, host, and substrate system should be studied separately for establishment of the most favorable conditions for inoculum production.
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spelling Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutionsGlomus etunicatumGigaspora margaritaScutellospora heterogamainoculum productionnutrient solutionThe production of inoculum is one of the hindrances in the large scale application of arbuscular mycorrhizal fungi (AMF). The objective of this work was to evaluate the effect of nutrient solutions with or without Tris-HCl buffer, on sporulation of AMF. The experiment was carried out in a greenhouse, using a substrate with sand and vermiculite (1:1 v/v). Fifty spores of Gigaspora margarita, Scutellospora heterogama, and Glomus etunicatum were inoculated in Sorghum vulgare (sorghum) or Panicum miliaceum (fodder millet). The substrate received the following nutrient solutions: Hoagland with 3 muM P (S1); Long Ashton II with 15.9 muM P (S2) and Hoagland with 20 muM P (S3), with or without 50 mM of Tris-HCl buffer (pH 6.5); the control treatment, consisting of a soil + sand + vermiculite (2:1:1 v/v) substrate was irrigated with deionized water. Ten weeks after the beginning of the experiment sporulation did not differ in treatments with sorghum. Panicum miliaceum promoted higher sporulation of the AMF than sorghum, and differences among treatments with nutrient solutions were observed. Production of spores of G. margarita and S. heterogama increased significantly after addition of buffer in S1 and S2, while that of G. etunicatum was improved when the substrate was irrigated with S1 + buffer and S3 solutions. Solution S1 + buffer benefited sporulation of the three fungi. However, as observed, each AMF, host, and substrate system should be studied separately for establishment of the most favorable conditions for inoculum production.Sociedade Brasileira de Microbiologia2005-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400004Brazilian Journal of Microbiology v.36 n.4 2005reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822005000400004info:eu-repo/semantics/openAccessSilva,Fábio Sérgio B.Yano-Melo,Adriana M.Brandão,Joana Angélica C.Maia,Leonor C.eng2006-05-10T00:00:00Zoai:scielo:S1517-83822005000400004Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-05-10T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
title Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
spellingShingle Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
Silva,Fábio Sérgio B.
Glomus etunicatum
Gigaspora margarita
Scutellospora heterogama
inoculum production
nutrient solution
title_short Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
title_full Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
title_fullStr Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
title_full_unstemmed Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
title_sort Sporulation of arbuscular mycorrhizal fungi using Tris-CHl buffer in addition to nutrient solutions
author Silva,Fábio Sérgio B.
author_facet Silva,Fábio Sérgio B.
Yano-Melo,Adriana M.
Brandão,Joana Angélica C.
Maia,Leonor C.
author_role author
author2 Yano-Melo,Adriana M.
Brandão,Joana Angélica C.
Maia,Leonor C.
author2_role author
author
author
dc.contributor.author.fl_str_mv Silva,Fábio Sérgio B.
Yano-Melo,Adriana M.
Brandão,Joana Angélica C.
Maia,Leonor C.
dc.subject.por.fl_str_mv Glomus etunicatum
Gigaspora margarita
Scutellospora heterogama
inoculum production
nutrient solution
topic Glomus etunicatum
Gigaspora margarita
Scutellospora heterogama
inoculum production
nutrient solution
description The production of inoculum is one of the hindrances in the large scale application of arbuscular mycorrhizal fungi (AMF). The objective of this work was to evaluate the effect of nutrient solutions with or without Tris-HCl buffer, on sporulation of AMF. The experiment was carried out in a greenhouse, using a substrate with sand and vermiculite (1:1 v/v). Fifty spores of Gigaspora margarita, Scutellospora heterogama, and Glomus etunicatum were inoculated in Sorghum vulgare (sorghum) or Panicum miliaceum (fodder millet). The substrate received the following nutrient solutions: Hoagland with 3 muM P (S1); Long Ashton II with 15.9 muM P (S2) and Hoagland with 20 muM P (S3), with or without 50 mM of Tris-HCl buffer (pH 6.5); the control treatment, consisting of a soil + sand + vermiculite (2:1:1 v/v) substrate was irrigated with deionized water. Ten weeks after the beginning of the experiment sporulation did not differ in treatments with sorghum. Panicum miliaceum promoted higher sporulation of the AMF than sorghum, and differences among treatments with nutrient solutions were observed. Production of spores of G. margarita and S. heterogama increased significantly after addition of buffer in S1 and S2, while that of G. etunicatum was improved when the substrate was irrigated with S1 + buffer and S3 solutions. Solution S1 + buffer benefited sporulation of the three fungi. However, as observed, each AMF, host, and substrate system should be studied separately for establishment of the most favorable conditions for inoculum production.
publishDate 2005
dc.date.none.fl_str_mv 2005-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000400004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822005000400004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.36 n.4 2005
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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