Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis

Detalhes bibliográficos
Autor(a) principal: Rosalem Junior,Wilson
Data de Publicação: 2006
Outros Autores: Souza,Rodrigo Carvalho de, Andrade,Arnaldo Feitosa Braga de, Colombo,Ana Paula Vieira
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006
Resumo: Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects. Whole saliva samples were collected for bacterial DNA isolation. The detection of Aa and the presence of the 530-bp genetic deletion were determined directly in the samples by polimerase chain reaction. Differences on clinical and microbiological parameters between the two groups were sought using the Mann-Whitney, Fisher´s exact and Chi-square tests. Associations between clinical and microbiological parameters were tested using the Pearson correlation coefficient. Aa was detected significantly more often in GAP patients (96.6%) than healthy subjects (76.7%) (chi2 = 4.9; p < 0.05). The genetic deletion was observed in 16 of the 28 (57.1%) GAP patients who were positive for Aa. However, none of the samples from individuals with periodontal health presented this deletion (chi2 = 19.15; p < 0.001). Strong correlations between the presence of the deletion and PPD (r=0.312, p<0.05), CAL (r=0.409, p<0.01), SB (r=0.278, p<0.05) and BOP (r=0.406, p<0.01) were found. A high frequency of Aa was observed in Brazilians with GAP and periodontal health. However, the highly leukotoxic genotype was observed only in subjects with generalized aggressive disease.
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spelling Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitisgeneralized aggressive periodontitisPCRActinobacillus actinomycetemcomitanssalivaleukotoxinActinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects. Whole saliva samples were collected for bacterial DNA isolation. The detection of Aa and the presence of the 530-bp genetic deletion were determined directly in the samples by polimerase chain reaction. Differences on clinical and microbiological parameters between the two groups were sought using the Mann-Whitney, Fisher´s exact and Chi-square tests. Associations between clinical and microbiological parameters were tested using the Pearson correlation coefficient. Aa was detected significantly more often in GAP patients (96.6%) than healthy subjects (76.7%) (chi2 = 4.9; p < 0.05). The genetic deletion was observed in 16 of the 28 (57.1%) GAP patients who were positive for Aa. However, none of the samples from individuals with periodontal health presented this deletion (chi2 = 19.15; p < 0.001). Strong correlations between the presence of the deletion and PPD (r=0.312, p<0.05), CAL (r=0.409, p<0.01), SB (r=0.278, p<0.05) and BOP (r=0.406, p<0.01) were found. A high frequency of Aa was observed in Brazilians with GAP and periodontal health. However, the highly leukotoxic genotype was observed only in subjects with generalized aggressive disease.Sociedade Brasileira de Microbiologia2006-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006Brazilian Journal of Microbiology v.37 n.2 2006reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822006000200006info:eu-repo/semantics/openAccessRosalem Junior,WilsonSouza,Rodrigo Carvalho deAndrade,Arnaldo Feitosa Braga deColombo,Ana Paula Vieiraeng2006-05-18T00:00:00Zoai:scielo:S1517-83822006000200006Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2006-05-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
title Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
spellingShingle Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
Rosalem Junior,Wilson
generalized aggressive periodontitis
PCR
Actinobacillus actinomycetemcomitans
saliva
leukotoxin
title_short Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
title_full Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
title_fullStr Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
title_full_unstemmed Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
title_sort Analysis of leukotoxin gene types of Actinobacillus actinomycetemcomitans in brazilians with aggressive periodontitis
author Rosalem Junior,Wilson
author_facet Rosalem Junior,Wilson
Souza,Rodrigo Carvalho de
Andrade,Arnaldo Feitosa Braga de
Colombo,Ana Paula Vieira
author_role author
author2 Souza,Rodrigo Carvalho de
Andrade,Arnaldo Feitosa Braga de
Colombo,Ana Paula Vieira
author2_role author
author
author
dc.contributor.author.fl_str_mv Rosalem Junior,Wilson
Souza,Rodrigo Carvalho de
Andrade,Arnaldo Feitosa Braga de
Colombo,Ana Paula Vieira
dc.subject.por.fl_str_mv generalized aggressive periodontitis
PCR
Actinobacillus actinomycetemcomitans
saliva
leukotoxin
topic generalized aggressive periodontitis
PCR
Actinobacillus actinomycetemcomitans
saliva
leukotoxin
description Actinobacillus actinomycetemcomitans (Aa) is considered a major etiologic agent of aggressive periodontitis but this species has also been associated with other forms of periodontal disease. Further, highly leukotoxic strains are related to severity of disease. The purpose of this study was to determine the prevalence of Aa and the occurrence of the leukotoxin gene 530-bp deletion in patients with generalized aggressive periodontitis (GAP) from a Brazilian population. Thirty periodontally healthy and 29 GAP subjects participated in the study. Full-mouth periodontal examination including probing pocket depth (PPD), clinical attachment level (CAL), supragingival biofilm (SB) and bleeding on probing (BOP) was carried out at 6 sites/tooth for all subjects. Whole saliva samples were collected for bacterial DNA isolation. The detection of Aa and the presence of the 530-bp genetic deletion were determined directly in the samples by polimerase chain reaction. Differences on clinical and microbiological parameters between the two groups were sought using the Mann-Whitney, Fisher´s exact and Chi-square tests. Associations between clinical and microbiological parameters were tested using the Pearson correlation coefficient. Aa was detected significantly more often in GAP patients (96.6%) than healthy subjects (76.7%) (chi2 = 4.9; p < 0.05). The genetic deletion was observed in 16 of the 28 (57.1%) GAP patients who were positive for Aa. However, none of the samples from individuals with periodontal health presented this deletion (chi2 = 19.15; p < 0.001). Strong correlations between the presence of the deletion and PPD (r=0.312, p<0.05), CAL (r=0.409, p<0.01), SB (r=0.278, p<0.05) and BOP (r=0.406, p<0.01) were found. A high frequency of Aa was observed in Brazilians with GAP and periodontal health. However, the highly leukotoxic genotype was observed only in subjects with generalized aggressive disease.
publishDate 2006
dc.date.none.fl_str_mv 2006-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822006000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.37 n.2 2006
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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