Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026 |
Resumo: | This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts. |
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Brazilian Journal of Microbiology |
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Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191chitinaseCellulosimicrobium cellulansfungal lysisprotoplastsThis study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts.Sociedade Brasileira de Microbiologia2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026Brazilian Journal of Microbiology v.40 n.3 2009reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822009000300026info:eu-repo/semantics/openAccessFleuri,Luciana F.Kawaguti,Haroldo Y.Sato,Hélia H.eng2009-08-18T00:00:00Zoai:scielo:S1517-83822009000300026Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-08-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
title |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
spellingShingle |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 Fleuri,Luciana F. chitinase Cellulosimicrobium cellulans fungal lysis protoplasts |
title_short |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
title_full |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
title_fullStr |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
title_full_unstemmed |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
title_sort |
Production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans 191 |
author |
Fleuri,Luciana F. |
author_facet |
Fleuri,Luciana F. Kawaguti,Haroldo Y. Sato,Hélia H. |
author_role |
author |
author2 |
Kawaguti,Haroldo Y. Sato,Hélia H. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Fleuri,Luciana F. Kawaguti,Haroldo Y. Sato,Hélia H. |
dc.subject.por.fl_str_mv |
chitinase Cellulosimicrobium cellulans fungal lysis protoplasts |
topic |
chitinase Cellulosimicrobium cellulans fungal lysis protoplasts |
description |
This study concerned the production, purification and application of extracellular chitinase from Cellulosimicrobium cellulans strain 191. In shaken flasks the maximum yield of chitinase was 6.9 U/mL after 72 h of cultivation at 25ºC and 200 rpm. In a 5 L fermenter with 1.5 vvm aeration, the highest yield obtained was 4.19 U/mL after 168 h of fermentation at 25ºC and 200 rpm, and using 3 vvm, it was 4.38 U/mL after 144 h of fermentation. The chitinase (61 KDa) was purified about 6.65 times by Sepharose CL 4B 200 gel filtration with a yield of 46.61%. The purified enzyme was able to lyse the cell walls of some fungi and to form protoplasts. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300026 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822009000300026 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.40 n.3 2009 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122202588708864 |