Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains

Detalhes bibliográficos
Autor(a) principal: Ferraz,Priscila N.
Data de Publicação: 2003
Outros Autores: Danelli,Maria das Graças M.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300011
Resumo: Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between the strains, being the major difference close to the 75 kDa level where a prominent peptide band was detected only in the F vaccine strain. Polyclonal antibodies to the M. gallisepticum antigens produced in chickens were used in serological tests for this antigenic variability study. There were strong cross-reactions between the strains and homologous/ heterologous antibodies. The most evident characteristic was the specific response of the vaccine-type F polyclonal antiserum to the 75 kDa peptide band of the homologous strain.
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spelling Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strainsMycoplasma gallisepticumphenotypic variationantigenic variationPhenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between the strains, being the major difference close to the 75 kDa level where a prominent peptide band was detected only in the F vaccine strain. Polyclonal antibodies to the M. gallisepticum antigens produced in chickens were used in serological tests for this antigenic variability study. There were strong cross-reactions between the strains and homologous/ heterologous antibodies. The most evident characteristic was the specific response of the vaccine-type F polyclonal antiserum to the 75 kDa peptide band of the homologous strain.Sociedade Brasileira de Microbiologia2003-07-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300011Brazilian Journal of Microbiology v.34 n.3 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000300011info:eu-repo/semantics/openAccessFerraz,Priscila N.Danelli,Maria das Graças M.eng2004-08-04T00:00:00Zoai:scielo:S1517-83822003000300011Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-08-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
title Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
spellingShingle Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
Ferraz,Priscila N.
Mycoplasma gallisepticum
phenotypic variation
antigenic variation
title_short Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
title_full Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
title_fullStr Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
title_full_unstemmed Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
title_sort Phenotypic and antigenic variation of Mycoplasma Gallisepticum vaccine strains
author Ferraz,Priscila N.
author_facet Ferraz,Priscila N.
Danelli,Maria das Graças M.
author_role author
author2 Danelli,Maria das Graças M.
author2_role author
dc.contributor.author.fl_str_mv Ferraz,Priscila N.
Danelli,Maria das Graças M.
dc.subject.por.fl_str_mv Mycoplasma gallisepticum
phenotypic variation
antigenic variation
topic Mycoplasma gallisepticum
phenotypic variation
antigenic variation
description Phenotypic and antigenic variations among Mycoplasma gallisepticum vaccine F and Ts-11 strains were investigated by SDS-PAGE and serological methods (hemagglutination-inhibition and immunoblot assays). The SDS-PAGE system followed by densitometer analysis showed weak phenotypic variability between the strains, being the major difference close to the 75 kDa level where a prominent peptide band was detected only in the F vaccine strain. Polyclonal antibodies to the M. gallisepticum antigens produced in chickens were used in serological tests for this antigenic variability study. There were strong cross-reactions between the strains and homologous/ heterologous antibodies. The most evident characteristic was the specific response of the vaccine-type F polyclonal antiserum to the 75 kDa peptide band of the homologous strain.
publishDate 2003
dc.date.none.fl_str_mv 2003-07-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300011
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000300011
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000300011
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 n.3 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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