Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage
Autor(a) principal: | |
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Data de Publicação: | 2012 |
Outros Autores: | , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400047 |
Resumo: | Samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the colonies. Considering the effect of metals on the respiratory enzymes, one strain of Ag-tolerantBacillus sp. and one of Hg-tolerant P. aeruginosa were unable to produce oxidase in the presence of Ag and Hg, respectively, while the expression of gelatinase was largely inhibited in various Gram negative strains (66% by Cr). Drug resistance in Hg-tolerant Enterobacteriaceae strains isolated from the university hospital sewage was greater than 80%, with prevalence of multiple resistance, while the Ag-tolerant strains from the same source showed about 34% of resistance, with the predominance of mono-resistance. Our results showed that, despite the ability of metal tolerant strains to survive and grow in the presence of these elements, the interactions with these metals may result in metabolic or phisiological changes in this group of bacteria. |
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Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewagemetal tolerancesewagepigmentenzymatic inhibitionantimicrobial resistanceSamples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the colonies. Considering the effect of metals on the respiratory enzymes, one strain of Ag-tolerantBacillus sp. and one of Hg-tolerant P. aeruginosa were unable to produce oxidase in the presence of Ag and Hg, respectively, while the expression of gelatinase was largely inhibited in various Gram negative strains (66% by Cr). Drug resistance in Hg-tolerant Enterobacteriaceae strains isolated from the university hospital sewage was greater than 80%, with prevalence of multiple resistance, while the Ag-tolerant strains from the same source showed about 34% of resistance, with the predominance of mono-resistance. Our results showed that, despite the ability of metal tolerant strains to survive and grow in the presence of these elements, the interactions with these metals may result in metabolic or phisiological changes in this group of bacteria.Sociedade Brasileira de Microbiologia2012-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400047Brazilian Journal of Microbiology v.43 n.4 2012reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822012000400047info:eu-repo/semantics/openAccessLima e Silva,Agostinho A. deCarvalho,Márcia A. Ribeiro deSouza,Sérgio A. L deDias,Patrícia M. TeixeiraSilva Filho,Renato G. daSaramago,Carmen S. de MeirellesBento,Cleonice A. de MeloHofer,Ernestoeng2013-02-19T00:00:00Zoai:scielo:S1517-83822012000400047Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2013-02-19T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
title |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
spellingShingle |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage Lima e Silva,Agostinho A. de metal tolerance sewage pigment enzymatic inhibition antimicrobial resistance |
title_short |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
title_full |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
title_fullStr |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
title_full_unstemmed |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
title_sort |
Heavy metal tolerance (Cr, Ag and Hg) in bacteria isolated from sewage |
author |
Lima e Silva,Agostinho A. de |
author_facet |
Lima e Silva,Agostinho A. de Carvalho,Márcia A. Ribeiro de Souza,Sérgio A. L de Dias,Patrícia M. Teixeira Silva Filho,Renato G. da Saramago,Carmen S. de Meirelles Bento,Cleonice A. de Melo Hofer,Ernesto |
author_role |
author |
author2 |
Carvalho,Márcia A. Ribeiro de Souza,Sérgio A. L de Dias,Patrícia M. Teixeira Silva Filho,Renato G. da Saramago,Carmen S. de Meirelles Bento,Cleonice A. de Melo Hofer,Ernesto |
author2_role |
author author author author author author author |
dc.contributor.author.fl_str_mv |
Lima e Silva,Agostinho A. de Carvalho,Márcia A. Ribeiro de Souza,Sérgio A. L de Dias,Patrícia M. Teixeira Silva Filho,Renato G. da Saramago,Carmen S. de Meirelles Bento,Cleonice A. de Melo Hofer,Ernesto |
dc.subject.por.fl_str_mv |
metal tolerance sewage pigment enzymatic inhibition antimicrobial resistance |
topic |
metal tolerance sewage pigment enzymatic inhibition antimicrobial resistance |
description |
Samples of sewage from a university hospital and a chemistry technical school were analysed for the percentage of bacterial tolerance to chromium (Cr), silver (Ag) and mercury (Hg). Additionally, we investigated the effect of these metals on pigmentation and on some enzymatic activities of the metal tolerant strains isolated, as well as antimicrobial resistance in some metal tolerant Enterobacteriaceae strains. Tolerance to Cr was observed mainly in Gram positive bacteria while in the case of Ag and Hg the tolerant bacteria were predominately Gram negative. Hg was the metal for which the percentage of tolerance was significantly higher, especially in samples from the hospital sewage (4.1%). Mercury also had the most discernible effect on color of the colonies. Considering the effect of metals on the respiratory enzymes, one strain of Ag-tolerantBacillus sp. and one of Hg-tolerant P. aeruginosa were unable to produce oxidase in the presence of Ag and Hg, respectively, while the expression of gelatinase was largely inhibited in various Gram negative strains (66% by Cr). Drug resistance in Hg-tolerant Enterobacteriaceae strains isolated from the university hospital sewage was greater than 80%, with prevalence of multiple resistance, while the Ag-tolerant strains from the same source showed about 34% of resistance, with the predominance of mono-resistance. Our results showed that, despite the ability of metal tolerant strains to survive and grow in the presence of these elements, the interactions with these metals may result in metabolic or phisiological changes in this group of bacteria. |
publishDate |
2012 |
dc.date.none.fl_str_mv |
2012-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400047 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000400047 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822012000400047 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.43 n.4 2012 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122205098999808 |