The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64

Detalhes bibliográficos
Autor(a) principal: Lv,Yuntong
Data de Publicação: 2016
Outros Autores: Xia,Zhikuan, Wang,Wenling
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100266
Resumo: Abstract The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers were present at the tip of the budding site of the spore, blastospore, and the germ tube of T. asahii. We also found that Spitzenkörpers were present at the tip of the hyphae as well as the subapical regions. Cytochalasin D, an inhibitor of actin polymerization, leads to abnormal Spitzenkörper formation and loss of cell polarity.
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spelling The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64Trichosporon asahiiSpitzenkörperPolarized growthCytochalasin DAbstract The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers were present at the tip of the budding site of the spore, blastospore, and the germ tube of T. asahii. We also found that Spitzenkörpers were present at the tip of the hyphae as well as the subapical regions. Cytochalasin D, an inhibitor of actin polymerization, leads to abnormal Spitzenkörper formation and loss of cell polarity.Sociedade Brasileira de Microbiologia2016-03-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100266Brazilian Journal of Microbiology v.47 n.1 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2015.11.002info:eu-repo/semantics/openAccessLv,YuntongXia,ZhikuanWang,Wenlingeng2016-03-01T00:00:00Zoai:scielo:S1517-83822016000100266Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-03-01T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
title The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
spellingShingle The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
Lv,Yuntong
Trichosporon asahii
Spitzenkörper
Polarized growth
Cytochalasin D
title_short The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
title_full The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
title_fullStr The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
title_full_unstemmed The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
title_sort The dynamic structure of Spitzenkörpers of Trichosporon asahii examined by the fluorescent probe FM4-64
author Lv,Yuntong
author_facet Lv,Yuntong
Xia,Zhikuan
Wang,Wenling
author_role author
author2 Xia,Zhikuan
Wang,Wenling
author2_role author
author
dc.contributor.author.fl_str_mv Lv,Yuntong
Xia,Zhikuan
Wang,Wenling
dc.subject.por.fl_str_mv Trichosporon asahii
Spitzenkörper
Polarized growth
Cytochalasin D
topic Trichosporon asahii
Spitzenkörper
Polarized growth
Cytochalasin D
description Abstract The Spitzenkörper is a dynamic and specialized multicomponent cell complex present in the tips of hyphal cells. The amphiphilic styryl dye FM4-64 was found to be ideal for imaging the dynamic changes of the apical vesicle cluster within growing hyphal tips. It is widely used as a marker of endocytosis and to visualize vacuolar membranes. Here we performed uptake experiments using FM4-64 to study the dynamic of the Spitzenkörper in Trichosporon asahii. We observed that Spitzenkörpers were present at the tip of the budding site of the spore, blastospore, and the germ tube of T. asahii. We also found that Spitzenkörpers were present at the tip of the hyphae as well as the subapical regions. Cytochalasin D, an inhibitor of actin polymerization, leads to abnormal Spitzenkörper formation and loss of cell polarity.
publishDate 2016
dc.date.none.fl_str_mv 2016-03-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100266
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100266
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2015.11.002
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.47 n.1 2016
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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