An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum

Detalhes bibliográficos
Autor(a) principal: Marassi,Carla Dray
Data de Publicação: 2008
Outros Autores: Silva,Marley, Oelemann,Walter Martin Roland, Fonseca,Leila de Souza, Ristow,Paula, Lilenbaum,Walter
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019
Resumo: ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
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spelling An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitumMycobacteriaELISAM. fortuitumM. phleiELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300019info:eu-repo/semantics/openAccessMarassi,Carla DraySilva,MarleyOelemann,Walter Martin RolandFonseca,Leila de SouzaRistow,PaulaLilenbaum,Waltereng2008-10-03T00:00:00Zoai:scielo:S1517-83822008000300019Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
spellingShingle An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
Marassi,Carla Dray
Mycobacteria
ELISA
M. fortuitum
M. phlei
title_short An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_full An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_fullStr An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_full_unstemmed An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
title_sort An alternative for the preadsorption step in the paratuberculosis serodiagnosis: Mycobacterium fortuitum
author Marassi,Carla Dray
author_facet Marassi,Carla Dray
Silva,Marley
Oelemann,Walter Martin Roland
Fonseca,Leila de Souza
Ristow,Paula
Lilenbaum,Walter
author_role author
author2 Silva,Marley
Oelemann,Walter Martin Roland
Fonseca,Leila de Souza
Ristow,Paula
Lilenbaum,Walter
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Marassi,Carla Dray
Silva,Marley
Oelemann,Walter Martin Roland
Fonseca,Leila de Souza
Ristow,Paula
Lilenbaum,Walter
dc.subject.por.fl_str_mv Mycobacteria
ELISA
M. fortuitum
M. phlei
topic Mycobacteria
ELISA
M. fortuitum
M. phlei
description ELISAs for paratuberculosis employ a preadsorption step with Mycobacterium phlei to diminish unspecific reactions As M. fortuitum is one of the most frequent environmental mycobacteria, the purpose of this pilot study was to evaluate its use as an alternative for the preadsorption in ELISAs for paratuberculosis. Results suggest that M. fortuitum can be an alternative instead of or associated to M. phlei with comparable results (κ > 0.8) to conventional ELISAs using M. phlei as a preadsorption antigen.
publishDate 2008
dc.date.none.fl_str_mv 2008-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300019
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822008000300019
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.39 n.3 2008
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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