ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells
Autor(a) principal: | |
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Data de Publicação: | 2003 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008 |
Resumo: | To ascertain the role of ExoU in late P. aeruginosa cytotoxicity, endothelial cells (EC) were exposed to wild type PA103, PA103deltaexoU and PA103::exsA for 1h and to gentamicin in culture medium. After 24h, the viability of PA103-infected cells (33.7 ± 14.3%) was significantly lower than the viability of PA103deltaexoU- (77.7 ± 6.3%) or PA103::exsA- (79.5 ± 23.3%) infected EC. P. aeruginosa cytotoxicity did not depend on the bacterial ability to interact with EC because the percentage of cells with associated PA103 (35.9 ± 15.8%) was similar to the percentage in PA103deltaexoU- (34.2 ± 16.0%) and lower than the percentage in PA103::exsA-infected cultures (82.9 ± 18.9%). Cell treatment with cytochalasin D reduced the PA103 internalization by EC but did not interfere with its ability to kill host cells. |
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Brazilian Journal of Microbiology |
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ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cellsType III secretion systemExoUendothelial cellsbacterial cytotoxicityTo ascertain the role of ExoU in late P. aeruginosa cytotoxicity, endothelial cells (EC) were exposed to wild type PA103, PA103deltaexoU and PA103::exsA for 1h and to gentamicin in culture medium. After 24h, the viability of PA103-infected cells (33.7 ± 14.3%) was significantly lower than the viability of PA103deltaexoU- (77.7 ± 6.3%) or PA103::exsA- (79.5 ± 23.3%) infected EC. P. aeruginosa cytotoxicity did not depend on the bacterial ability to interact with EC because the percentage of cells with associated PA103 (35.9 ± 15.8%) was similar to the percentage in PA103deltaexoU- (34.2 ± 16.0%) and lower than the percentage in PA103::exsA-infected cultures (82.9 ± 18.9%). Cell treatment with cytochalasin D reduced the PA103 internalization by EC but did not interfere with its ability to kill host cells.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500008info:eu-repo/semantics/openAccessSaliba,Alessandra MattosNishi,RebecaAssis,Maria CristinaPlotkowski,Maria Cristinaeng2004-11-29T00:00:00Zoai:scielo:S1517-83822003000500008Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
title |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
spellingShingle |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells Saliba,Alessandra Mattos Type III secretion system ExoU endothelial cells bacterial cytotoxicity |
title_short |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
title_full |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
title_fullStr |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
title_full_unstemmed |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
title_sort |
ExoU contributes to late killing of Pseudomonas aeruginosa - infected endothelial cells |
author |
Saliba,Alessandra Mattos |
author_facet |
Saliba,Alessandra Mattos Nishi,Rebeca Assis,Maria Cristina Plotkowski,Maria Cristina |
author_role |
author |
author2 |
Nishi,Rebeca Assis,Maria Cristina Plotkowski,Maria Cristina |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Saliba,Alessandra Mattos Nishi,Rebeca Assis,Maria Cristina Plotkowski,Maria Cristina |
dc.subject.por.fl_str_mv |
Type III secretion system ExoU endothelial cells bacterial cytotoxicity |
topic |
Type III secretion system ExoU endothelial cells bacterial cytotoxicity |
description |
To ascertain the role of ExoU in late P. aeruginosa cytotoxicity, endothelial cells (EC) were exposed to wild type PA103, PA103deltaexoU and PA103::exsA for 1h and to gentamicin in culture medium. After 24h, the viability of PA103-infected cells (33.7 ± 14.3%) was significantly lower than the viability of PA103deltaexoU- (77.7 ± 6.3%) or PA103::exsA- (79.5 ± 23.3%) infected EC. P. aeruginosa cytotoxicity did not depend on the bacterial ability to interact with EC because the percentage of cells with associated PA103 (35.9 ± 15.8%) was similar to the percentage in PA103deltaexoU- (34.2 ± 16.0%) and lower than the percentage in PA103::exsA-infected cultures (82.9 ± 18.9%). Cell treatment with cytochalasin D reduced the PA103 internalization by EC but did not interfere with its ability to kill host cells. |
publishDate |
2003 |
dc.date.none.fl_str_mv |
2003-11-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500008 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822003000500008 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.34 suppl.1 2003 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199740776448 |