Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans

Detalhes bibliográficos
Autor(a) principal: Barense,Renata I.
Data de Publicação: 2001
Outros Autores: Chellegatti,Maria Angélica dos S.C., Fonseca,Maria José V., Said,Suraia
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014
Resumo: Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
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spelling Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentansExopolygalacturonasepectinasepolygalacturonasePenicillium frequentansPrevious studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.Sociedade Brasileira de Microbiologia2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014Brazilian Journal of Microbiology v.32 n.4 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000400014info:eu-repo/semantics/openAccessBarense,Renata I.Chellegatti,Maria Angélica dos S.C.Fonseca,Maria José V.Said,Suraiaeng2002-09-02T00:00:00Zoai:scielo:S1517-83822001000400014Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-09-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
title Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
spellingShingle Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
Barense,Renata I.
Exopolygalacturonase
pectinase
polygalacturonase
Penicillium frequentans
title_short Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
title_full Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
title_fullStr Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
title_full_unstemmed Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
title_sort Partial purification and characterization of exopolygalacturonase II and III of Penicillium frequentans
author Barense,Renata I.
author_facet Barense,Renata I.
Chellegatti,Maria Angélica dos S.C.
Fonseca,Maria José V.
Said,Suraia
author_role author
author2 Chellegatti,Maria Angélica dos S.C.
Fonseca,Maria José V.
Said,Suraia
author2_role author
author
author
dc.contributor.author.fl_str_mv Barense,Renata I.
Chellegatti,Maria Angélica dos S.C.
Fonseca,Maria José V.
Said,Suraia
dc.subject.por.fl_str_mv Exopolygalacturonase
pectinase
polygalacturonase
Penicillium frequentans
topic Exopolygalacturonase
pectinase
polygalacturonase
Penicillium frequentans
description Previous studies from our laboratory have demonstrated that the fungus Penicillium frequentans produces high levels of polygalacturonase and pectinesterase. Endopolygalacturonase I (Endo-PG I) and Exopolygalacturonase I (Exo-PG I) were previously purified and characterized. In the present study two extracellular polygalacturonases were separated, partially purified and biochemically characterized. Both were characterized as exopolygalacturonases so they were named exopolygalacturonase II (Exo-PG II) and exopolygalacturonase III (Exo-PG III) which had a molecular mass of 63 kDa (Exo-PG II) and 79 kDa (Exo-PG III). The Km values were 1.6 and 0.059 g/L and the Vmax values were 2571 and 185 U/mg, respectively. The optimum temperature was 50ºC for both enzymes, while the optimum pH was 5.0 for Exo-PG II and 5.8 for Exo-PG III.
publishDate 2001
dc.date.none.fl_str_mv 2001-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400014
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822001000400014
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.32 n.4 2001
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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