Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers

Detalhes bibliográficos
Autor(a) principal: Brasileiro,Bereneuza Tavares Ramos Valente
Data de Publicação: 2004
Outros Autores: Coimbra,Maria Raquel Moura, Morais Jr,Marcos Antonio de, Oliveira,Neiva Tinti de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006
Resumo: Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical origin and substrate. Its polymorphism was observed even in the very conserved sequence of rDNA locus, and the SPAR marker (GTG)5 showed the highest polymorphism. Together, those results may contribute to understand the relation between fungal genetic variability and cultivars resistance phenotypes to fungal-caused diseases, helping plant-breeding programs.
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spelling Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markersintron splice siteFusarium solanimicrosatellitePCR-fingerprintingribosomal DNAFusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical origin and substrate. Its polymorphism was observed even in the very conserved sequence of rDNA locus, and the SPAR marker (GTG)5 showed the highest polymorphism. Together, those results may contribute to understand the relation between fungal genetic variability and cultivars resistance phenotypes to fungal-caused diseases, helping plant-breeding programs.Sociedade Brasileira de Microbiologia2004-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006Brazilian Journal of Microbiology v.35 n.3 2004reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822004000200006info:eu-repo/semantics/openAccessBrasileiro,Bereneuza Tavares Ramos ValenteCoimbra,Maria Raquel MouraMorais Jr,Marcos Antonio deOliveira,Neiva Tinti deeng2005-11-25T00:00:00Zoai:scielo:S1517-83822004000200006Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2005-11-25T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
title Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
spellingShingle Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
Brasileiro,Bereneuza Tavares Ramos Valente
intron splice site
Fusarium solani
microsatellite
PCR-fingerprinting
ribosomal DNA
title_short Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
title_full Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
title_fullStr Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
title_full_unstemmed Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
title_sort Genetic variability within Fusarium solani specie as revealed by PCR-fingerprinting based on pcr markers
author Brasileiro,Bereneuza Tavares Ramos Valente
author_facet Brasileiro,Bereneuza Tavares Ramos Valente
Coimbra,Maria Raquel Moura
Morais Jr,Marcos Antonio de
Oliveira,Neiva Tinti de
author_role author
author2 Coimbra,Maria Raquel Moura
Morais Jr,Marcos Antonio de
Oliveira,Neiva Tinti de
author2_role author
author
author
dc.contributor.author.fl_str_mv Brasileiro,Bereneuza Tavares Ramos Valente
Coimbra,Maria Raquel Moura
Morais Jr,Marcos Antonio de
Oliveira,Neiva Tinti de
dc.subject.por.fl_str_mv intron splice site
Fusarium solani
microsatellite
PCR-fingerprinting
ribosomal DNA
topic intron splice site
Fusarium solani
microsatellite
PCR-fingerprinting
ribosomal DNA
description Fusarium solani fungus (teleomorph Haematonectria haematococca) is of relevance for agriculture, producing a disease that causes significant losses for many cultivars. Moreover, F. solani is an opportunistic pathogen to animals and humans. The complexity associated to its correct identification by traditional methods justifies the efforts of using molecular markers for isolates characterization. In this work, three PCR-based methods (one PCR-ribotyping and two PCR-fingerprinting) were used to investigate the molecular variability of eighteen F. solani isolates from four Brazilian States, collected from different substrates. Genetic analysis revealed the intraspecific variability within the F. solani isolates, without any correlation to their geographical origin and substrate. Its polymorphism was observed even in the very conserved sequence of rDNA locus, and the SPAR marker (GTG)5 showed the highest polymorphism. Together, those results may contribute to understand the relation between fungal genetic variability and cultivars resistance phenotypes to fungal-caused diseases, helping plant-breeding programs.
publishDate 2004
dc.date.none.fl_str_mv 2004-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200006
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822004000200006
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.35 n.3 2004
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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