Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever
Autor(a) principal: | |
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Data de Publicação: | 2014 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400040 |
Resumo: | The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae. |
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Brazilian Journal of Microbiology |
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Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric FeverBrazilian purpuric feverHaemophilus influenzae biogroup aegyptiusgenetic transferNeisseria meningitidisHaemophilus influenzaeThe Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae.Sociedade Brasileira de Microbiologia2014-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400040Brazilian Journal of Microbiology v.45 n.4 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000400040info:eu-repo/semantics/openAccessCury,Gisele Cristiane GentilePereira,Rafaella Fabiana CarneiroHollanda,Luciana Maria deLancellotti,Marceloeng2015-02-13T00:00:00Zoai:scielo:S1517-83822014000400040Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-02-13T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
title |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
spellingShingle |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever Cury,Gisele Cristiane Gentile Brazilian purpuric fever Haemophilus influenzae biogroup aegyptius genetic transfer Neisseria meningitidis Haemophilus influenzae |
title_short |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
title_full |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
title_fullStr |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
title_full_unstemmed |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
title_sort |
Inflammatory response of Haemophilus influenzae biotype aegyptius causing Brazilian Purpuric Fever |
author |
Cury,Gisele Cristiane Gentile |
author_facet |
Cury,Gisele Cristiane Gentile Pereira,Rafaella Fabiana Carneiro Hollanda,Luciana Maria de Lancellotti,Marcelo |
author_role |
author |
author2 |
Pereira,Rafaella Fabiana Carneiro Hollanda,Luciana Maria de Lancellotti,Marcelo |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cury,Gisele Cristiane Gentile Pereira,Rafaella Fabiana Carneiro Hollanda,Luciana Maria de Lancellotti,Marcelo |
dc.subject.por.fl_str_mv |
Brazilian purpuric fever Haemophilus influenzae biogroup aegyptius genetic transfer Neisseria meningitidis Haemophilus influenzae |
topic |
Brazilian purpuric fever Haemophilus influenzae biogroup aegyptius genetic transfer Neisseria meningitidis Haemophilus influenzae |
description |
The Brazilian Purpuric Fever (BPF) is a systemic disease with many clinical features of meningococcal sepsis and is usually preceded by purulent conjunctivitis. The illness is caused by Haemophilus influenza biogroup aegyptius, which was associated exclusively with conjunctivitis. In this work construction of the las gene, hypothetically responsible for this virulence, were fusioned with ermAM cassette in Neisseria meningitidis virulent strains and had its DNA transfer to non BPF H. influenzae strains. The effect of the las transfer was capable to increase the cytokines TNFα and IL10 expression in Hec-1B cells line infected with these transformed mutants (in eight log scale of folding change RNA expression). This is the first molecular study involving the las transfer to search an elucidation of the pathogenic factors by horizontal intergeneric transfer from meningococci to H. influenzae. |
publishDate |
2014 |
dc.date.none.fl_str_mv |
2014-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400040 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822014000400040 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.45 n.4 2014 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122206962319360 |