Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile

Detalhes bibliográficos
Autor(a) principal: Matter,Letícia B.
Data de Publicação: 2015
Outros Autores: Spricigo,Denis A., Tasca,Caiane, de Vargas,Agueda C.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300875
Resumo: The invasin gimB (genetic island associated with human newborn meningitis) is usually found in ExPEC (Extraintestinal Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitis E. coli) and APEC (avian pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a zoonotic agent and the scarce information about the frequency of gimB-carrying strains in different animal species, the aim of this study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli SB278/94 from a bovine specimen was positive (1/47) for gimB, which represents 2.1% of the bovine isolates. The ability of SB278/94 to adhere to and invade eukaryotic cells was confirmed by adherence and gentamicin-protection assays using HeLa cells. This is the first study that investigates for gimB in bovine, canine and feline E. coli isolates and shows E. coli from the intestinal-bovine samples harboring gimB.
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spelling Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profilegimBadherenceinvasivenesszoonotic potentiallivestockcompanion animalsThe invasin gimB (genetic island associated with human newborn meningitis) is usually found in ExPEC (Extraintestinal Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitis E. coli) and APEC (avian pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a zoonotic agent and the scarce information about the frequency of gimB-carrying strains in different animal species, the aim of this study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli SB278/94 from a bovine specimen was positive (1/47) for gimB, which represents 2.1% of the bovine isolates. The ability of SB278/94 to adhere to and invade eukaryotic cells was confirmed by adherence and gentamicin-protection assays using HeLa cells. This is the first study that investigates for gimB in bovine, canine and feline E. coli isolates and shows E. coli from the intestinal-bovine samples harboring gimB.Sociedade Brasileira de Microbiologia2015-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300875Brazilian Journal of Microbiology v.46 n.3 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246320140621info:eu-repo/semantics/openAccessMatter,Letícia B.Spricigo,Denis A.Tasca,Caianede Vargas,Agueda C.eng2015-08-31T00:00:00Zoai:scielo:S1517-83822015000300875Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-08-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
title Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
spellingShingle Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
Matter,Letícia B.
gimB
adherence
invasiveness
zoonotic potential
livestock
companion animals
title_short Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
title_full Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
title_fullStr Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
title_full_unstemmed Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
title_sort Invasin gimB found in a bovine intestinal Escherichia coli with an adherent and invasive profile
author Matter,Letícia B.
author_facet Matter,Letícia B.
Spricigo,Denis A.
Tasca,Caiane
de Vargas,Agueda C.
author_role author
author2 Spricigo,Denis A.
Tasca,Caiane
de Vargas,Agueda C.
author2_role author
author
author
dc.contributor.author.fl_str_mv Matter,Letícia B.
Spricigo,Denis A.
Tasca,Caiane
de Vargas,Agueda C.
dc.subject.por.fl_str_mv gimB
adherence
invasiveness
zoonotic potential
livestock
companion animals
topic gimB
adherence
invasiveness
zoonotic potential
livestock
companion animals
description The invasin gimB (genetic island associated with human newborn meningitis) is usually found in ExPEC (Extraintestinal Pathogenic Escherichia coli) such as UPEC (uropathogenic E. coli), NMEC (neonatal meningitis E. coli) and APEC (avian pathogenic E. coli). In NMEC, gimB is associated with the invasion process of the host cells. Due to the importance of E. coli as a zoonotic agent and the scarce information about the frequency of gimB-carrying strains in different animal species, the aim of this study was to investigate the presence of gimB in isolates from bovine, swine, canine and feline clinical samples. PCR was conducted on 196 isolates and the identity of the amplicons was confirmed by sequencing. Of the samples tested, only E. coli SB278/94 from a bovine specimen was positive (1/47) for gimB, which represents 2.1% of the bovine isolates. The ability of SB278/94 to adhere to and invade eukaryotic cells was confirmed by adherence and gentamicin-protection assays using HeLa cells. This is the first study that investigates for gimB in bovine, canine and feline E. coli isolates and shows E. coli from the intestinal-bovine samples harboring gimB.
publishDate 2015
dc.date.none.fl_str_mv 2015-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
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status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300875
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000300875
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-838246320140621
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.46 n.3 2015
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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