Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2

Detalhes bibliográficos
Autor(a) principal: Sangeetha,R.
Data de Publicação: 2014
Outros Autores: Arulpandi,I., Geetha,A.
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200004
Resumo: Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have been made inaccessible by dimerisation, thus rendering the lipase resistant to protease.
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spelling Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2Bacillus pumiluscovalent dimerlipaseproteolytic-resistanceProteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have been made inaccessible by dimerisation, thus rendering the lipase resistant to protease.Sociedade Brasileira de Microbiologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200004Brazilian Journal of Microbiology v.45 n.2 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000200004info:eu-repo/semantics/openAccessSangeetha,R.Arulpandi,I.Geetha,A.eng2014-09-30T00:00:00Zoai:scielo:S1517-83822014000200004Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-09-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
title Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
spellingShingle Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
Sangeetha,R.
Bacillus pumilus
covalent dimer
lipase
proteolytic-resistance
title_short Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
title_full Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
title_fullStr Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
title_full_unstemmed Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
title_sort Molecular characterization of a proteolysis-resistant lipase from Bacillus pumilus SG2
author Sangeetha,R.
author_facet Sangeetha,R.
Arulpandi,I.
Geetha,A.
author_role author
author2 Arulpandi,I.
Geetha,A.
author2_role author
author
dc.contributor.author.fl_str_mv Sangeetha,R.
Arulpandi,I.
Geetha,A.
dc.subject.por.fl_str_mv Bacillus pumilus
covalent dimer
lipase
proteolytic-resistance
topic Bacillus pumilus
covalent dimer
lipase
proteolytic-resistance
description Proteolysis-resistant lipases can be well exploited by industrial processes which employ both lipase and protease as biocatalysts. A proteolysis resistant lipase from Bacillus pumilus SG2 was isolated, purified and characterized earlier. The lipase was resistant to native and commercial proteases. In the present work, we have characterized the lip gene which encodes the proteolysis-resistant lipase from Bacillus pumilus SG2. The parameters and structural details of lipase were analysed. The lip gene consisted of 650 bp. The experimental molecular weight of SG2 lipase was nearly double that of its theoretical molecular weight, thus suggesting the existence of the functional lipase as a covalent dimer. The proteolytic cleavage sites of the lipase would have been made inaccessible by dimerisation, thus rendering the lipase resistant to protease.
publishDate 2014
dc.date.none.fl_str_mv 2014-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200004
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200004
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000200004
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.2 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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