Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao
Autor(a) principal: | |
---|---|
Data de Publicação: | 2008 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300010 |
Resumo: | In spite of the acknowledged importance of growth-promoting bacteria, only a reduced number of studies were conducted with these microorganisms on Theobroma cacao. The objectives of this work were to study the population densities and genetic diversity of actinomycetes associated with the rhizosphere of cacao as a first step in their application in plant growth promotion and biological control. The populations densities of actinomycetes in soil and cacao roots were similar, with mean values of 1,0 x 10(6) CFU/g and 9,6 x 10(5) CFU/g, respectively. All isolates selected and used in this study were identified through sequencing analyses of a fragment of the rpoB gene that encodes the β-subunit of the RNA polymerase as species of the genus Streptomyces. In vitro cellulolytic, xilanolytic and chitinolytic activity, indolacetic acid production and phosphate solubilization activities were observed in most of the isolates tested. The data obtained in this study demonstrate that actinomycetes account for a higher percentage of the total population of culturable bacteria in soil than on cacao roots. Additionally, actinomycetes from the cacao rhizosphere are genetically diverse and have potential applications as agents of growth promotion. |
id |
SBM-1_8c15f5c9f7829c0e0ba945290dcb0bc7 |
---|---|
oai_identifier_str |
oai:scielo:S1517-83822008000300010 |
network_acronym_str |
SBM-1 |
network_name_str |
Brazilian Journal of Microbiology |
repository_id_str |
|
spelling |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacaocacaorpoB sequence analysesStreptomyces sppIn spite of the acknowledged importance of growth-promoting bacteria, only a reduced number of studies were conducted with these microorganisms on Theobroma cacao. The objectives of this work were to study the population densities and genetic diversity of actinomycetes associated with the rhizosphere of cacao as a first step in their application in plant growth promotion and biological control. The populations densities of actinomycetes in soil and cacao roots were similar, with mean values of 1,0 x 10(6) CFU/g and 9,6 x 10(5) CFU/g, respectively. All isolates selected and used in this study were identified through sequencing analyses of a fragment of the rpoB gene that encodes the β-subunit of the RNA polymerase as species of the genus Streptomyces. In vitro cellulolytic, xilanolytic and chitinolytic activity, indolacetic acid production and phosphate solubilization activities were observed in most of the isolates tested. The data obtained in this study demonstrate that actinomycetes account for a higher percentage of the total population of culturable bacteria in soil than on cacao roots. Additionally, actinomycetes from the cacao rhizosphere are genetically diverse and have potential applications as agents of growth promotion.Sociedade Brasileira de Microbiologia2008-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300010Brazilian Journal of Microbiology v.39 n.3 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000300010info:eu-repo/semantics/openAccessBarreto,Tâmara R.Silva,Augusto C.M. daSoares,Ana Cristina F.Souza,Jorge T. deeng2008-10-08T00:00:00Zoai:scielo:S1517-83822008000300010Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2008-10-08T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
title |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
spellingShingle |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao Barreto,Tâmara R. cacao rpoB sequence analyses Streptomyces spp |
title_short |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
title_full |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
title_fullStr |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
title_full_unstemmed |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
title_sort |
Population densities and genetic diversity of actinomycetes associated to the rhizosphere of Theobroma cacao |
author |
Barreto,Tâmara R. |
author_facet |
Barreto,Tâmara R. Silva,Augusto C.M. da Soares,Ana Cristina F. Souza,Jorge T. de |
author_role |
author |
author2 |
Silva,Augusto C.M. da Soares,Ana Cristina F. Souza,Jorge T. de |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Barreto,Tâmara R. Silva,Augusto C.M. da Soares,Ana Cristina F. Souza,Jorge T. de |
dc.subject.por.fl_str_mv |
cacao rpoB sequence analyses Streptomyces spp |
topic |
cacao rpoB sequence analyses Streptomyces spp |
description |
In spite of the acknowledged importance of growth-promoting bacteria, only a reduced number of studies were conducted with these microorganisms on Theobroma cacao. The objectives of this work were to study the population densities and genetic diversity of actinomycetes associated with the rhizosphere of cacao as a first step in their application in plant growth promotion and biological control. The populations densities of actinomycetes in soil and cacao roots were similar, with mean values of 1,0 x 10(6) CFU/g and 9,6 x 10(5) CFU/g, respectively. All isolates selected and used in this study were identified through sequencing analyses of a fragment of the rpoB gene that encodes the β-subunit of the RNA polymerase as species of the genus Streptomyces. In vitro cellulolytic, xilanolytic and chitinolytic activity, indolacetic acid production and phosphate solubilization activities were observed in most of the isolates tested. The data obtained in this study demonstrate that actinomycetes account for a higher percentage of the total population of culturable bacteria in soil than on cacao roots. Additionally, actinomycetes from the cacao rhizosphere are genetically diverse and have potential applications as agents of growth promotion. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300010 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300010 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000300010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.3 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122201811714048 |