An improved purification procedure for Leishmania RNA virus (LRV)
| Autor(a) principal: | |
|---|---|
| Data de Publicação: | 2014 |
| Outros Autores: | , , , , , , |
| Tipo de documento: | Artigo |
| Idioma: | eng |
| Título da fonte: | Brazilian Journal of Microbiology |
| Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044 |
Resumo: | Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations. |
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Brazilian Journal of Microbiology |
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An improved purification procedure for Leishmania RNA virus (LRV)Leishmania guyanensisLeishmania RNA virusLRVvirus isolationelectron microscopyLeishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations.Sociedade Brasileira de Microbiologia2014-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044Brazilian Journal of Microbiology v.45 n.2 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000200044info:eu-repo/semantics/openAccessSouza,Marcos Michel deManzine,Livia ReginaSilva,Marcos Vinicius G. daBettini,JeffersonPortugal,Rodrigo VilaresCruz,Angela KayselArruda,EuricoThiemann,Otavio Henriqueeng2014-09-30T00:00:00Zoai:scielo:S1517-83822014000200044Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-09-30T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
| dc.title.none.fl_str_mv |
An improved purification procedure for Leishmania RNA virus (LRV) |
| title |
An improved purification procedure for Leishmania RNA virus (LRV) |
| spellingShingle |
An improved purification procedure for Leishmania RNA virus (LRV) Souza,Marcos Michel de Leishmania guyanensis Leishmania RNA virus LRV virus isolation electron microscopy |
| title_short |
An improved purification procedure for Leishmania RNA virus (LRV) |
| title_full |
An improved purification procedure for Leishmania RNA virus (LRV) |
| title_fullStr |
An improved purification procedure for Leishmania RNA virus (LRV) |
| title_full_unstemmed |
An improved purification procedure for Leishmania RNA virus (LRV) |
| title_sort |
An improved purification procedure for Leishmania RNA virus (LRV) |
| author |
Souza,Marcos Michel de |
| author_facet |
Souza,Marcos Michel de Manzine,Livia Regina Silva,Marcos Vinicius G. da Bettini,Jefferson Portugal,Rodrigo Vilares Cruz,Angela Kaysel Arruda,Eurico Thiemann,Otavio Henrique |
| author_role |
author |
| author2 |
Manzine,Livia Regina Silva,Marcos Vinicius G. da Bettini,Jefferson Portugal,Rodrigo Vilares Cruz,Angela Kaysel Arruda,Eurico Thiemann,Otavio Henrique |
| author2_role |
author author author author author author author |
| dc.contributor.author.fl_str_mv |
Souza,Marcos Michel de Manzine,Livia Regina Silva,Marcos Vinicius G. da Bettini,Jefferson Portugal,Rodrigo Vilares Cruz,Angela Kaysel Arruda,Eurico Thiemann,Otavio Henrique |
| dc.subject.por.fl_str_mv |
Leishmania guyanensis Leishmania RNA virus LRV virus isolation electron microscopy |
| topic |
Leishmania guyanensis Leishmania RNA virus LRV virus isolation electron microscopy |
| description |
Leishmania RNA Virus (LRV, Totiviridae) infect Leishmania cells and subvert mice immune response, probably promoting parasite persistence, suggesting significant roles for LRV in host-parasite interaction. Here we describe a new LRV1-4 purification protocol, enabling capsid visualization by negatively stained electron microscopy representing a significant contribution to future LRV investigations. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014-06-01 |
| dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
| dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044 |
| url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000200044 |
| dc.language.iso.fl_str_mv |
eng |
| language |
eng |
| dc.relation.none.fl_str_mv |
10.1590/S1517-83822014000200044 |
| dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
text/html |
| dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
| publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
| dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.45 n.2 2014 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
| instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
| instacron_str |
SBM |
| institution |
SBM |
| reponame_str |
Brazilian Journal of Microbiology |
| collection |
Brazilian Journal of Microbiology |
| repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
| repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
| _version_ |
1752122206549180416 |