Benzene, toluene and xylene biodegradation by Pseudomonas putida CCMI 852

• Autor(a) principal: Otenio,Marcelo Henrique
• Data de Publicação: 2005
• Outros Autores: Silva,Maria Teresa Lopes da, Marques,Maria Luiza Oliveira, Roseiro,José Carlos, Bidoia,Ederio Dino
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822005000300010
• topic: Pseudomonas putida; biodegradation; BTX; plasmid TOL

A minimal liquid medium containing benzene (B), toluene (T) and xylene (X) and mixtures thereof, was used to evaluate degradation activity of Pseudomonas putida CCMI 852 containing a TOL plasmid. Experiments were developed with B, T and X (100 mg L-1), with mixtures of BT, BX, and TX (50 + 50 mg L-1 each) and BTX (33.3 + 33.3 + 33.3 mg L-1 each), added to 500 mL of medium. After 18 to 24 hours, the inoculum was added and solvent disappearance was determined after 24 to 25 hours by GC. Results showed that P. putida CCMI 852 was able to metabolize T and X, but B was not metabolized. In a BTX mixture, B was not metabolized and T and X degradation rate decreased 50%.