Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests
Autor(a) principal: | |
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Data de Publicação: | 2011 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040 |
Resumo: | West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells. |
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Brazilian Journal of Microbiology |
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Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic testsWest Nile virusWNVM/E glycoproteinsstable expressiondiagnosticWest Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells.Sociedade Brasileira de Microbiologia2011-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040Brazilian Journal of Microbiology v.42 n.3 2011reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822011000300040info:eu-repo/semantics/openAccessCargnelutti,Juliana FelipettoBrum,Mário Celso SperottoWeiblen,RudiFlores,Eduardo Furtadoeng2011-12-21T00:00:00Zoai:scielo:S1517-83822011000300040Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2011-12-21T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
title |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
spellingShingle |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests Cargnelutti,Juliana Felipetto West Nile virus WNV M/E glycoproteins stable expression diagnostic |
title_short |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
title_full |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
title_fullStr |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
title_full_unstemmed |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
title_sort |
Stable expression and potential use of West Nile virus envelope glycoproteins preM/E as antigen in diagnostic tests |
author |
Cargnelutti,Juliana Felipetto |
author_facet |
Cargnelutti,Juliana Felipetto Brum,Mário Celso Sperotto Weiblen,Rudi Flores,Eduardo Furtado |
author_role |
author |
author2 |
Brum,Mário Celso Sperotto Weiblen,Rudi Flores,Eduardo Furtado |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Cargnelutti,Juliana Felipetto Brum,Mário Celso Sperotto Weiblen,Rudi Flores,Eduardo Furtado |
dc.subject.por.fl_str_mv |
West Nile virus WNV M/E glycoproteins stable expression diagnostic |
topic |
West Nile virus WNV M/E glycoproteins stable expression diagnostic |
description |
West Nile virus (WNV) envelope glycoproteins preM/E were stably expressed in baby hamster kidney cells and tested as antigen in a fluorescent antibody assay for WNV antibodies. Sera from horses, mice and chicken immunized with an inactivated WNV vaccine and, less consistently, sera from horses acutely infected with WNV, reacted specifically with viral antigens present in preM/E-expressing cells. |
publishDate |
2011 |
dc.date.none.fl_str_mv |
2011-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300040 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822011000300040 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.42 n.3 2011 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122203918303232 |