Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers
Autor(a) principal: | |
---|---|
Data de Publicação: | 2001 |
Outros Autores: | , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400013 |
Resumo: | Aiming at contributing to technological improvements in plant fiber processing methods, this paper reports research work on the obtainment of more efficient pectinase-producing fungi strains. More specifically, this work reports the analysis of 18 strains of filamentous fungi, with the purpose of obtaining enzymes for textile fibers degumming. The strains were evaluated for production of pectinolytic enzymes under several growth conditions (culture medium and growth temperature). Production of pectinases was measured by an enzymatic index (EI) in solid pectin medium. Among the tested strains, Penicillium chrysogenum IFO 4626 (Q 176) showed the best performance. Genetic improvement of this strain was carried out to increase its pectinase production, while keeping cellulase activity down to a negligible level, since cellulases are known to decrease the resistance of the fiber. Variability was induced through several cycles of mutation and selection by exposing conidea to ultra-violet light (UV). We selected 39 out of 390 isolated colonies. Resulting mutants produced nine times more pectin lyase (PL) than the original strain in terms of PL specific activity, and five times more in terms of PL activity (i.e. mmoles liberated per minute of reaction per mL of medium). Periodically, mutant performance was evaluated in solid pectin medium. Genetic stability was maintained for four years after isolation. |
id |
SBM-1_9bfbe5946a5d556ba5812a385a30800b |
---|---|
oai_identifier_str |
oai:scielo:S1517-83822001000400013 |
network_acronym_str |
SBM-1 |
network_name_str |
Brazilian Journal of Microbiology |
repository_id_str |
|
spelling |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibersPenicillium chrysogenumpectin lyasepectinolytic enzymedegumming of textile fibersAiming at contributing to technological improvements in plant fiber processing methods, this paper reports research work on the obtainment of more efficient pectinase-producing fungi strains. More specifically, this work reports the analysis of 18 strains of filamentous fungi, with the purpose of obtaining enzymes for textile fibers degumming. The strains were evaluated for production of pectinolytic enzymes under several growth conditions (culture medium and growth temperature). Production of pectinases was measured by an enzymatic index (EI) in solid pectin medium. Among the tested strains, Penicillium chrysogenum IFO 4626 (Q 176) showed the best performance. Genetic improvement of this strain was carried out to increase its pectinase production, while keeping cellulase activity down to a negligible level, since cellulases are known to decrease the resistance of the fiber. Variability was induced through several cycles of mutation and selection by exposing conidea to ultra-violet light (UV). We selected 39 out of 390 isolated colonies. Resulting mutants produced nine times more pectin lyase (PL) than the original strain in terms of PL specific activity, and five times more in terms of PL activity (i.e. mmoles liberated per minute of reaction per mL of medium). Periodically, mutant performance was evaluated in solid pectin medium. Genetic stability was maintained for four years after isolation.Sociedade Brasileira de Microbiologia2001-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400013Brazilian Journal of Microbiology v.32 n.4 2001reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822001000400013info:eu-repo/semantics/openAccessMolina,Silvia M.G.Pelissari,Francisco A.Vitorello,Cláudia B.M.eng2002-09-02T00:00:00Zoai:scielo:S1517-83822001000400013Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2002-09-02T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
title |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
spellingShingle |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers Molina,Silvia M.G. Penicillium chrysogenum pectin lyase pectinolytic enzyme degumming of textile fibers |
title_short |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
title_full |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
title_fullStr |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
title_full_unstemmed |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
title_sort |
Screening and genetic improvement of pectinolytic fungi for degumming of textile fibers |
author |
Molina,Silvia M.G. |
author_facet |
Molina,Silvia M.G. Pelissari,Francisco A. Vitorello,Cláudia B.M. |
author_role |
author |
author2 |
Pelissari,Francisco A. Vitorello,Cláudia B.M. |
author2_role |
author author |
dc.contributor.author.fl_str_mv |
Molina,Silvia M.G. Pelissari,Francisco A. Vitorello,Cláudia B.M. |
dc.subject.por.fl_str_mv |
Penicillium chrysogenum pectin lyase pectinolytic enzyme degumming of textile fibers |
topic |
Penicillium chrysogenum pectin lyase pectinolytic enzyme degumming of textile fibers |
description |
Aiming at contributing to technological improvements in plant fiber processing methods, this paper reports research work on the obtainment of more efficient pectinase-producing fungi strains. More specifically, this work reports the analysis of 18 strains of filamentous fungi, with the purpose of obtaining enzymes for textile fibers degumming. The strains were evaluated for production of pectinolytic enzymes under several growth conditions (culture medium and growth temperature). Production of pectinases was measured by an enzymatic index (EI) in solid pectin medium. Among the tested strains, Penicillium chrysogenum IFO 4626 (Q 176) showed the best performance. Genetic improvement of this strain was carried out to increase its pectinase production, while keeping cellulase activity down to a negligible level, since cellulases are known to decrease the resistance of the fiber. Variability was induced through several cycles of mutation and selection by exposing conidea to ultra-violet light (UV). We selected 39 out of 390 isolated colonies. Resulting mutants produced nine times more pectin lyase (PL) than the original strain in terms of PL specific activity, and five times more in terms of PL activity (i.e. mmoles liberated per minute of reaction per mL of medium). Periodically, mutant performance was evaluated in solid pectin medium. Genetic stability was maintained for four years after isolation. |
publishDate |
2001 |
dc.date.none.fl_str_mv |
2001-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400013 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000400013 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822001000400013 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.32 n.4 2001 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122199219634176 |