Novel nonsense mutation in the katA gene of a catalase-negative Staphylococcus aureus strain

• Autor(a) principal: Lagos,Jaime
• Data de Publicação: 2016
• Outros Autores: Alarcón,Pedro, Benadof,Dona, Ulloa,Soledad, Fasce,Rodrigo, Tognarelli,Javier, Aguayo,Carolina, Araya,Pamela, Parra,Bárbara, Olivares,Berta, Hormazábal,Juan Carlos, Fernández,Jorge
• Tipo de documento: Artigo
• Idioma: eng
• Título da fonte: Brazilian Journal of Microbiology
• Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000100177
• topic: Staphylococcus aureus; Catalase-negative; Catalase gene; katA

Abstract We report the first description of a rare catalase-negative strain of Staphylococcus aureus in Chile. This new variant was isolated from blood and synovial tissue samples of a pediatric patient. Sequencing analysis revealed that this catalase-negative strain is related to ST10 strain, which has earlier been described in relation to S. aureus carriers. Interestingly, sequence analysis of the catalase gene katA revealed presence of a novel nonsense mutation that causes premature translational truncation of the C-terminus of the enzyme leading to a loss of 222 amino acids. Our study suggests that loss of catalase activity in this rare catalase-negative Chilean strain is due to this novel nonsense mutation in the katA gene, which truncates the enzyme to just 283 amino acids.