Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards
Autor(a) principal: | |
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Data de Publicação: | 2016 |
Outros Autores: | , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401030 |
Resumo: | Abstract An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter. |
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Brazilian Journal of Microbiology |
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Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchardsMango orchardArthrobacter kerguelensisS,S-dipropyl carbonodithioateAbstract An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter.Sociedade Brasileira de Microbiologia2016-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401030Brazilian Journal of Microbiology v.47 n.4 2016reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2016.07.010info:eu-repo/semantics/openAccessMunaganti,Rajesh KumarMuvva,VijayalakshmiKonda,SaiduluNaragani,KrishnaMangamuri,Usha KiranmayiDorigondla,Kumar ReddyAkkewar,Dattatray. M.eng2016-11-21T00:00:00Zoai:scielo:S1517-83822016000401030Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2016-11-21T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
title |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
spellingShingle |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards Munaganti,Rajesh Kumar Mango orchard Arthrobacter kerguelensis S,S-dipropyl carbonodithioate |
title_short |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
title_full |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
title_fullStr |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
title_full_unstemmed |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
title_sort |
Antimicrobial profile of Arthrobacter kerguelensis VL-RK_09 isolated from Mango orchards |
author |
Munaganti,Rajesh Kumar |
author_facet |
Munaganti,Rajesh Kumar Muvva,Vijayalakshmi Konda,Saidulu Naragani,Krishna Mangamuri,Usha Kiranmayi Dorigondla,Kumar Reddy Akkewar,Dattatray. M. |
author_role |
author |
author2 |
Muvva,Vijayalakshmi Konda,Saidulu Naragani,Krishna Mangamuri,Usha Kiranmayi Dorigondla,Kumar Reddy Akkewar,Dattatray. M. |
author2_role |
author author author author author author |
dc.contributor.author.fl_str_mv |
Munaganti,Rajesh Kumar Muvva,Vijayalakshmi Konda,Saidulu Naragani,Krishna Mangamuri,Usha Kiranmayi Dorigondla,Kumar Reddy Akkewar,Dattatray. M. |
dc.subject.por.fl_str_mv |
Mango orchard Arthrobacter kerguelensis S,S-dipropyl carbonodithioate |
topic |
Mango orchard Arthrobacter kerguelensis S,S-dipropyl carbonodithioate |
description |
Abstract An actinobacterial strain VL-RK_09 having potential antimicrobial activities was isolated from a mango orchard in Krishna District, Andhra Pradesh (India) and was identified as Arthrobacter kerguelensis. The strain A. kerguelensis VL-RK_09 exhibited a broad spectrum of in vitro antimicrobial activity against bacteria and fungi. Production of bioactive metabolites by the strain was the highest in modified yeast extract malt extract dextrose broth, as compared to other media tested. Lactose (1%) and peptone (0.5%) were found to be the most suitable carbon and nitrogen sources, respectively, for the optimum production of the bioactive metabolites. The maximum production of the bioactive metabolites was detected in the culture medium with an initial pH of 7, in which the strain was incubated for five days at 30 °C under shaking conditions. Screening of secondary metabolites obtained from the culture broth led to the isolation of a compound active against a wide variety of Gram-positive and negative bacteria and fungi. The structure of the first active fraction was elucidated using Fourier transform infrared spectroscopy, electrospray ionization mass spectrometry, 1H and 13C nuclear magnetic resonance spectroscopy. The compound was identified as S,S-dipropyl carbonodithioate. This study is the first report of the occurrence of this compound in the genus Arthrobacter. |
publishDate |
2016 |
dc.date.none.fl_str_mv |
2016-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401030 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822016000401030 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2016.07.010 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.47 n.4 2016 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
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1752122208787890176 |