Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties
Main Author: | |
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Publication Date: | 2008 |
Other Authors: | , , , , |
Format: | Article |
Language: | eng |
Source: | Brazilian Journal of Microbiology |
Download full: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023 |
Summary: | A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species. Neither effect of the plant part nor sugarcane variety was observed during the cluster analysis. The observed metabolic and genetic variability will be helpful during the strain selection studies for sugarcane inoculation in association with sugarcane breeding programs. |
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Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varietieshorizontal spreadresistance plasmidsESBLA polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species. Neither effect of the plant part nor sugarcane variety was observed during the cluster analysis. The observed metabolic and genetic variability will be helpful during the strain selection studies for sugarcane inoculation in association with sugarcane breeding programs.Sociedade Brasileira de Microbiologia2008-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023Brazilian Journal of Microbiology v.39 n.4 2008reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822008000400023info:eu-repo/semantics/openAccessGuedes,Helma V.Santos,Samuel T. dosPerin,LiamaraTeixeira,Kátia R. dos S.Reis,Veronica M.Baldani,José I.eng2009-04-06T00:00:00Zoai:scielo:S1517-83822008000400023Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-04-06T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
title |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
spellingShingle |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties Guedes,Helma V. horizontal spread resistance plasmids ESBL |
title_short |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
title_full |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
title_fullStr |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
title_full_unstemmed |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
title_sort |
Polyphasic characterization of Gluconacetobacterdiazotrophicus isolates obtained from different sugarcane varieties |
author |
Guedes,Helma V. |
author_facet |
Guedes,Helma V. Santos,Samuel T. dos Perin,Liamara Teixeira,Kátia R. dos S. Reis,Veronica M. Baldani,José I. |
author_role |
author |
author2 |
Santos,Samuel T. dos Perin,Liamara Teixeira,Kátia R. dos S. Reis,Veronica M. Baldani,José I. |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Guedes,Helma V. Santos,Samuel T. dos Perin,Liamara Teixeira,Kátia R. dos S. Reis,Veronica M. Baldani,José I. |
dc.subject.por.fl_str_mv |
horizontal spread resistance plasmids ESBL |
topic |
horizontal spread resistance plasmids ESBL |
description |
A polyphasic approach was applied to characterize 35 G. diazotrophicus isolates obtained from sugarcane varieties cultivated in Brazil. The isolates were analyzed by phenotypic (use of different carbon sources) and genotypic tests (ARDRA and RISARFLP techniques). Variability among the isolates was observed in relation to the carbon source use preference. Glucose and sucrose were used by all isolates in contrast to myo-inositol, galactose and ribose that were not metabolized. The results of the analysis showed the presence of two groups clustered at 68% of similarity. The genetic distance was higher when RISA-RFLP analysis was used. Analysis of 16S rDNA sequences from isolates showed that all of them belonged to the G. diazotrophicus species. Neither effect of the plant part nor sugarcane variety was observed during the cluster analysis. The observed metabolic and genetic variability will be helpful during the strain selection studies for sugarcane inoculation in association with sugarcane breeding programs. |
publishDate |
2008 |
dc.date.none.fl_str_mv |
2008-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000400023 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822008000400023 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.39 n.4 2008 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122202171375616 |