Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189
Autor(a) principal: | |
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Data de Publicação: | 2015 |
Outros Autores: | , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401147 |
Resumo: | Abstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent. |
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Brazilian Journal of Microbiology |
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Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189bacteriocinpyocinP. aeruginosaAbstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent.Sociedade Brasileira de Microbiologia2015-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401147Brazilian Journal of Microbiology v.46 n.4 2015reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-838246420140737info:eu-repo/semantics/openAccessNaz,Sehar AfshanJabeen,NusratSohail,MuhammadRasool,Sheikh Ajazeng2015-12-17T00:00:00Zoai:scielo:S1517-83822015000401147Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2015-12-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
title |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
spellingShingle |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 Naz,Sehar Afshan bacteriocin pyocin P. aeruginosa |
title_short |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
title_full |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
title_fullStr |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
title_full_unstemmed |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
title_sort |
Biophysicochemical characterization of Pyocin SA189 produced by Pseudomonas aeruginosa SA189 |
author |
Naz,Sehar Afshan |
author_facet |
Naz,Sehar Afshan Jabeen,Nusrat Sohail,Muhammad Rasool,Sheikh Ajaz |
author_role |
author |
author2 |
Jabeen,Nusrat Sohail,Muhammad Rasool,Sheikh Ajaz |
author2_role |
author author author |
dc.contributor.author.fl_str_mv |
Naz,Sehar Afshan Jabeen,Nusrat Sohail,Muhammad Rasool,Sheikh Ajaz |
dc.subject.por.fl_str_mv |
bacteriocin pyocin P. aeruginosa |
topic |
bacteriocin pyocin P. aeruginosa |
description |
Abstract Pseudomonas aeruginosa, in spite of being a ubiquitous organism (as it is found in soil, water, and humans), is also an opportunistic pathogen. In order to maintain its diversity in the community, it produces various toxic proteins, known as, bacteriocins. In the present study, pyocin SA189, which is a bacteriocin produced by P. aeruginosa SA189 (isolated from a clinical sample) was characterized. P. aeruginosa SA189, as identified by the conventional and 16S rRNA gene amplification, produced pyocin SA189 of molecular weight of 66 k Da. The pyocin showed antimicrobial activity against several clinically relevant Gram-positive and Gram-negative bacteria and was substantially stable for wide ranges of temperature and pH. Furthermore, the pyocin also retained its biological activity upon treatment with metal ions, organic solvents, and various proteolytic and lipolytic enzymes. The data from the growth kinetics indicated that the maximum bacteriocin production occurred in the late log phase. Overall, our results signify the potential of pyocin SA189 as a bio-control agent. |
publishDate |
2015 |
dc.date.none.fl_str_mv |
2015-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401147 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822015000401147 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-838246420140737 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.46 n.4 2015 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122207882969088 |