Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria

Detalhes bibliográficos
Autor(a) principal: Muthukamalam,Santhakumar
Data de Publicação: 2017
Outros Autores: Sivagangavathi,Sivalingam, Dhrishya,Dharmapal, Sudha Rani,Sadras
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400637
Resumo: ABSTRACT Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O. Among the three strains, Microbacterium hydrocarbonoxydans exhibited superior crude oil degrading ability as evidenced by its superior growth rate in crude oil enriched medium and enhanced activity of dioxygenases. Also degradation of total petroleum hydrocarbon (TPH) in crude oil was higher with Microbacterium hydrocarbonoxydans. The three strains also produced biosurfactants of glycolipid nature as indicated d by biochemical, FTIR and GCMS analysis. These findings emphasize that such bacterial strains with superior oil degrading capacity may find their potential application in bioremediation of oil spills and conservation of marine and soil ecosystem.
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spelling Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteriaCrude oilBiodegradationBacteriaCatechol dioxygenaseBiosurfactantABSTRACT Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O. Among the three strains, Microbacterium hydrocarbonoxydans exhibited superior crude oil degrading ability as evidenced by its superior growth rate in crude oil enriched medium and enhanced activity of dioxygenases. Also degradation of total petroleum hydrocarbon (TPH) in crude oil was higher with Microbacterium hydrocarbonoxydans. The three strains also produced biosurfactants of glycolipid nature as indicated d by biochemical, FTIR and GCMS analysis. These findings emphasize that such bacterial strains with superior oil degrading capacity may find their potential application in bioremediation of oil spills and conservation of marine and soil ecosystem.Sociedade Brasileira de Microbiologia2017-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400637Brazilian Journal of Microbiology v.48 n.4 2017reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2017.02.007info:eu-repo/semantics/openAccessMuthukamalam,SanthakumarSivagangavathi,SivalingamDhrishya,DharmapalSudha Rani,Sadraseng2017-10-31T00:00:00Zoai:scielo:S1517-83822017000400637Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2017-10-31T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
title Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
spellingShingle Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
Muthukamalam,Santhakumar
Crude oil
Biodegradation
Bacteria
Catechol dioxygenase
Biosurfactant
title_short Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
title_full Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
title_fullStr Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
title_full_unstemmed Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
title_sort Characterization of dioxygenases and biosurfactants produced by crude oil degrading soil bacteria
author Muthukamalam,Santhakumar
author_facet Muthukamalam,Santhakumar
Sivagangavathi,Sivalingam
Dhrishya,Dharmapal
Sudha Rani,Sadras
author_role author
author2 Sivagangavathi,Sivalingam
Dhrishya,Dharmapal
Sudha Rani,Sadras
author2_role author
author
author
dc.contributor.author.fl_str_mv Muthukamalam,Santhakumar
Sivagangavathi,Sivalingam
Dhrishya,Dharmapal
Sudha Rani,Sadras
dc.subject.por.fl_str_mv Crude oil
Biodegradation
Bacteria
Catechol dioxygenase
Biosurfactant
topic Crude oil
Biodegradation
Bacteria
Catechol dioxygenase
Biosurfactant
description ABSTRACT Role of microbes in bioremediation of oil spills has become inevitable owing to their eco friendly nature. This study focused on the isolation and characterization of bacterial strains with superior oil degrading potential from crude-oil contaminated soil. Three such bacterial strains were selected and subsequently identified by 16S rRNA gene sequence analysis as Corynebacterium aurimucosum, Acinetobacter baumannii and Microbacterium hydrocarbonoxydans respectively. The specific activity of catechol 1,2 dioxygenase (C12O) and catechol 2,3 dioxygenase (C23O) was determined in these three strains wherein the activity of C12O was more than that of C23O. Among the three strains, Microbacterium hydrocarbonoxydans exhibited superior crude oil degrading ability as evidenced by its superior growth rate in crude oil enriched medium and enhanced activity of dioxygenases. Also degradation of total petroleum hydrocarbon (TPH) in crude oil was higher with Microbacterium hydrocarbonoxydans. The three strains also produced biosurfactants of glycolipid nature as indicated d by biochemical, FTIR and GCMS analysis. These findings emphasize that such bacterial strains with superior oil degrading capacity may find their potential application in bioremediation of oil spills and conservation of marine and soil ecosystem.
publishDate 2017
dc.date.none.fl_str_mv 2017-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400637
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000400637
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1016/j.bjm.2017.02.007
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.48 n.4 2017
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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