Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR

Detalhes bibliográficos
Autor(a) principal: Figueiredo,Eduardo Eustáquio de Souza
Data de Publicação: 2010
Outros Autores: Carvalho,Ricardo Cezar Tavares, Silvestre,Flávia Galindo, Lilenbaum,Walter, Fonseca,Leila Sousa, Silva,Joab Trajano, Paschoalin,Vânia Margaret Flosi
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
Resumo: Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
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spelling Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCRMycobacterium bovismultiplex-PCRnasal swabbovine tuberculosisDetection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.Sociedade Brasileira de Microbiologia2010-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020Brazilian Journal of Microbiology v.41 n.2 2010reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822010000200020info:eu-repo/semantics/openAccessFigueiredo,Eduardo Eustáquio de SouzaCarvalho,Ricardo Cezar TavaresSilvestre,Flávia GalindoLilenbaum,WalterFonseca,Leila SousaSilva,Joab TrajanoPaschoalin,Vânia Margaret Flosieng2010-04-16T00:00:00Zoai:scielo:S1517-83822010000200020Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2010-04-16T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
spellingShingle Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
Figueiredo,Eduardo Eustáquio de Souza
Mycobacterium bovis
multiplex-PCR
nasal swab
bovine tuberculosis
title_short Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_full Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_fullStr Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_full_unstemmed Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
title_sort Detection of Mycobacterium bovis DNA in nasal swabs from tuberculous cattle by a multiplex PCR
author Figueiredo,Eduardo Eustáquio de Souza
author_facet Figueiredo,Eduardo Eustáquio de Souza
Carvalho,Ricardo Cezar Tavares
Silvestre,Flávia Galindo
Lilenbaum,Walter
Fonseca,Leila Sousa
Silva,Joab Trajano
Paschoalin,Vânia Margaret Flosi
author_role author
author2 Carvalho,Ricardo Cezar Tavares
Silvestre,Flávia Galindo
Lilenbaum,Walter
Fonseca,Leila Sousa
Silva,Joab Trajano
Paschoalin,Vânia Margaret Flosi
author2_role author
author
author
author
author
author
dc.contributor.author.fl_str_mv Figueiredo,Eduardo Eustáquio de Souza
Carvalho,Ricardo Cezar Tavares
Silvestre,Flávia Galindo
Lilenbaum,Walter
Fonseca,Leila Sousa
Silva,Joab Trajano
Paschoalin,Vânia Margaret Flosi
dc.subject.por.fl_str_mv Mycobacterium bovis
multiplex-PCR
nasal swab
bovine tuberculosis
topic Mycobacterium bovis
multiplex-PCR
nasal swab
bovine tuberculosis
description Detection of tuberculosis in cattle relies on the intradermal tuberculin test (ITT), but a definitive diagnosis requires identification of the pathogen after the animal is slaughtered. DNA in nasal swabs from 50 cows was analyzed by m-PCR, targeting for the RvD1-Rv2031c and IS6110 sequences. M. bovis was identified in two of 34 tuberculous cows (5.9%). The use of mPCR of nasal swabs as an in vivo diagnostic tool for bovine tuberculosis is suggested.
publishDate 2010
dc.date.none.fl_str_mv 2010-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822010000200020
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822010000200020
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.41 n.2 2010
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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