Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process
Autor(a) principal: | |
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Data de Publicação: | 2018 |
Outros Autores: | , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400749 |
Resumo: | ABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation. The experimental design resulted in an efficient pyrene degradation, reducing the experiment time while the PAH concentration applied in the assays was increased. The selected fungus was able to degrade almost 100% of pyrene (0.08 mg mL-1) after 48 h of incubation under saline condition, without generating toxic compounds and with a TOC reduction of 17%. Intermediate metabolites of pyrene degradation were identified, suggesting that the fungus degraded the compound via the cytochrome P450 system and epoxide hydrolases. These results highlight the relevance of marine-derived fungi in the field of PAH bioremediation, adding value to the blue biotechnology. |
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Brazilian Journal of Microbiology |
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Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation processMarine biotechnologyPAHsFungiAcute toxicityExperimental designABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation. The experimental design resulted in an efficient pyrene degradation, reducing the experiment time while the PAH concentration applied in the assays was increased. The selected fungus was able to degrade almost 100% of pyrene (0.08 mg mL-1) after 48 h of incubation under saline condition, without generating toxic compounds and with a TOC reduction of 17%. Intermediate metabolites of pyrene degradation were identified, suggesting that the fungus degraded the compound via the cytochrome P450 system and epoxide hydrolases. These results highlight the relevance of marine-derived fungi in the field of PAH bioremediation, adding value to the blue biotechnology.Sociedade Brasileira de Microbiologia2018-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400749Brazilian Journal of Microbiology v.49 n.4 2018reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1016/j.bjm.2018.04.007info:eu-repo/semantics/openAccessVieira,Gabriela A.L.Magrini,Mariana JuventinaBonugli-Santos,Rafaella C.Rodrigues,Marili V.N.Sette,Lara D.eng2018-10-17T00:00:00Zoai:scielo:S1517-83822018000400749Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2018-10-17T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
title |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
spellingShingle |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process Vieira,Gabriela A.L. Marine biotechnology PAHs Fungi Acute toxicity Experimental design |
title_short |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
title_full |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
title_fullStr |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
title_full_unstemmed |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
title_sort |
Polycyclic aromatic hydrocarbons degradation by marine-derived basidiomycetes: optimization of the degradation process |
author |
Vieira,Gabriela A.L. |
author_facet |
Vieira,Gabriela A.L. Magrini,Mariana Juventina Bonugli-Santos,Rafaella C. Rodrigues,Marili V.N. Sette,Lara D. |
author_role |
author |
author2 |
Magrini,Mariana Juventina Bonugli-Santos,Rafaella C. Rodrigues,Marili V.N. Sette,Lara D. |
author2_role |
author author author author |
dc.contributor.author.fl_str_mv |
Vieira,Gabriela A.L. Magrini,Mariana Juventina Bonugli-Santos,Rafaella C. Rodrigues,Marili V.N. Sette,Lara D. |
dc.subject.por.fl_str_mv |
Marine biotechnology PAHs Fungi Acute toxicity Experimental design |
topic |
Marine biotechnology PAHs Fungi Acute toxicity Experimental design |
description |
ABSTRACT Pyrene and benzo[a]pyrene (BaP) are high molecular weight polycyclic aromatic hydrocarbons (PAHs) recalcitrant to microbial attack. Although studies related to the microbial degradation of PAHs have been carried out in the last decades, little is known about degradation of these environmental pollutants by fungi from marine origin. Therefore, this study aimed to select one PAHs degrader among three marine-derived basidiomycete fungi and to study its pyrene detoxification/degradation. Marasmiellus sp. CBMAI 1062 showed higher levels of pyrene and BaP degradation and was subjected to studies related to pyrene degradation optimization using experimental design, acute toxicity, organic carbon removal (TOC), and metabolite evaluation. The experimental design resulted in an efficient pyrene degradation, reducing the experiment time while the PAH concentration applied in the assays was increased. The selected fungus was able to degrade almost 100% of pyrene (0.08 mg mL-1) after 48 h of incubation under saline condition, without generating toxic compounds and with a TOC reduction of 17%. Intermediate metabolites of pyrene degradation were identified, suggesting that the fungus degraded the compound via the cytochrome P450 system and epoxide hydrolases. These results highlight the relevance of marine-derived fungi in the field of PAH bioremediation, adding value to the blue biotechnology. |
publishDate |
2018 |
dc.date.none.fl_str_mv |
2018-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400749 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000400749 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1016/j.bjm.2018.04.007 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.49 n.4 2018 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122209762017280 |