Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires

Detalhes bibliográficos
Autor(a) principal: Seyffert,Núbia
Data de Publicação: 2003
Outros Autores: Silva,Éverton Fagonde da, Bermudez,Viviane Lorenzato, Brod,Claudiomar Soares
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025
Resumo: In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.
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spelling Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospiresLeptospiramicroscopic agglutinationLeptospirosislaboratorial diagnosisIn leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.Sociedade Brasileira de Microbiologia2003-11-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025Brazilian Journal of Microbiology v.34 suppl.1 2003reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822003000500025info:eu-repo/semantics/openAccessSeyffert,NúbiaSilva,Éverton Fagonde daBermudez,Viviane LorenzatoBrod,Claudiomar Soareseng2004-11-29T00:00:00Zoai:scielo:S1517-83822003000500025Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2004-11-29T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
title Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
spellingShingle Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
Seyffert,Núbia
Leptospira
microscopic agglutination
Leptospirosis
laboratorial diagnosis
title_short Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
title_full Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
title_fullStr Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
title_full_unstemmed Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
title_sort Influence of temperature, incubation time, antigen density and age of the culture in agglutinability of leptospires
author Seyffert,Núbia
author_facet Seyffert,Núbia
Silva,Éverton Fagonde da
Bermudez,Viviane Lorenzato
Brod,Claudiomar Soares
author_role author
author2 Silva,Éverton Fagonde da
Bermudez,Viviane Lorenzato
Brod,Claudiomar Soares
author2_role author
author
author
dc.contributor.author.fl_str_mv Seyffert,Núbia
Silva,Éverton Fagonde da
Bermudez,Viviane Lorenzato
Brod,Claudiomar Soares
dc.subject.por.fl_str_mv Leptospira
microscopic agglutination
Leptospirosis
laboratorial diagnosis
topic Leptospira
microscopic agglutination
Leptospirosis
laboratorial diagnosis
description In leptospirosis, the Microscopic Agglutination Test (MAT) is the basis of serological diagnosis and the most widely used. In our study, the MAT was applied to evaluate the influence of temperature, incubation time, antigen density and age of the culture in the agglutinability of Leptospires and the interference of these factors in diagnosis. Three serum samples with titers of 100, 800 and 25.600 to the serovar tande (local isolate) of canicola serogroup were used with different combinations among the four factors. There was a significant relation among all factors, specially between the antigen density and age of the culture.
publishDate 2003
dc.date.none.fl_str_mv 2003-11-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000500025
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822003000500025
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.34 suppl.1 2003
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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