Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk

Detalhes bibliográficos
Autor(a) principal: Martins,Maurilio L.
Data de Publicação: 2014
Outros Autores: Uelinton,M. Pinto, Riedel,Kathrin, Vanetti,Maria C.D., Mantovani,Hilário C., Araújo,Elza F. de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037
Resumo: Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains.
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spelling Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milkmilkPseudomonas fluorescensbiofilm formationquorum sensingquorum quenchingNumerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains.Sociedade Brasileira de Microbiologia2014-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037Brazilian Journal of Microbiology v.45 n.3 2014reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822014000300037info:eu-repo/semantics/openAccessMartins,Maurilio L.Uelinton,M. PintoRiedel,KathrinVanetti,Maria C.D.Mantovani,Hilário C.Araújo,Elza F. deeng2014-11-04T00:00:00Zoai:scielo:S1517-83822014000300037Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-11-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
spellingShingle Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
Martins,Maurilio L.
milk
Pseudomonas fluorescens
biofilm formation
quorum sensing
quorum quenching
title_short Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_full Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_fullStr Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_full_unstemmed Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
title_sort Lack of AHL-based quorum sensing in Pseudomonas fluorescens isolated from milk
author Martins,Maurilio L.
author_facet Martins,Maurilio L.
Uelinton,M. Pinto
Riedel,Kathrin
Vanetti,Maria C.D.
Mantovani,Hilário C.
Araújo,Elza F. de
author_role author
author2 Uelinton,M. Pinto
Riedel,Kathrin
Vanetti,Maria C.D.
Mantovani,Hilário C.
Araújo,Elza F. de
author2_role author
author
author
author
author
dc.contributor.author.fl_str_mv Martins,Maurilio L.
Uelinton,M. Pinto
Riedel,Kathrin
Vanetti,Maria C.D.
Mantovani,Hilário C.
Araújo,Elza F. de
dc.subject.por.fl_str_mv milk
Pseudomonas fluorescens
biofilm formation
quorum sensing
quorum quenching
topic milk
Pseudomonas fluorescens
biofilm formation
quorum sensing
quorum quenching
description Numerous bacteria coordinate gene expression in response to small signalling molecules in many cases known as acylhomoserine lactones (AHLs), which accumulate as a function of cell density in a process known as quorum sensing. This work aimed to determine if phenotypes that are important to define microbial activity in foods such as biofilm formation, swarming motility and proteolytic activity of two Pseudomonas fluorescens strains, isolated from refrigerated raw milk, are influenced by AHL molecules. The tested P. fluorescens strains did not produce AHL molecules in none of the evaluated media. We found that biofilm formation was dependent on the culture media, but it was not influenced by AHLs. Our results indicate that biofilm formation, swarming motility and proteolytic activity of the tested P. fluorescens strains are not regulated by acyl-homoserine lactones. It is likely that AHL-dependent quorum sensing system is absent from these strains.
publishDate 2014
dc.date.none.fl_str_mv 2014-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300037
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822014000300037
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.45 n.3 2014
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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