Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola

Detalhes bibliográficos
Autor(a) principal: Takahashi,Jacqueline Aparecida
Data de Publicação: 2000
Outros Autores: Barroso,Henrique de Almeida, Oliveira,Alaíde Braga de
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200003
Resumo: Parameters for a more efficient biotransformation of diterpene-like compounds by the fungus Cephalosporium aphidicola were established by carrying out microscale feedings at several conditions. Experiments were guided by thin layer chromatography and gas chromatography analysis. It was observed that the substrate should be added in ethanol at concentrations between 15 to 30 mg per 100 ml of medium. The extraction of the product showed to be more efficient when carried out from both mycelia and broth and using ethylacetate as the extracting solvent. The experiment should be stopped six days after feeding the substrate to the fungus for the best product yield.
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spelling Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicolaCephalosporium aphidicolabiotransformationditerpeneParameters for a more efficient biotransformation of diterpene-like compounds by the fungus Cephalosporium aphidicola were established by carrying out microscale feedings at several conditions. Experiments were guided by thin layer chromatography and gas chromatography analysis. It was observed that the substrate should be added in ethanol at concentrations between 15 to 30 mg per 100 ml of medium. The extraction of the product showed to be more efficient when carried out from both mycelia and broth and using ethylacetate as the extracting solvent. The experiment should be stopped six days after feeding the substrate to the fungus for the best product yield.Sociedade Brasileira de Microbiologia2000-06-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200003Brazilian Journal of Microbiology v.31 n.2 2000reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822000000200003info:eu-repo/semantics/openAccessTakahashi,Jacqueline AparecidaBarroso,Henrique de AlmeidaOliveira,Alaíde Braga deeng2000-10-04T00:00:00Zoai:scielo:S1517-83822000000200003Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2000-10-04T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
title Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
spellingShingle Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
Takahashi,Jacqueline Aparecida
Cephalosporium aphidicola
biotransformation
diterpene
title_short Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
title_full Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
title_fullStr Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
title_full_unstemmed Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
title_sort Optimization of diterpenes bioconversion process by the fungus Cephalosporium aphidicola
author Takahashi,Jacqueline Aparecida
author_facet Takahashi,Jacqueline Aparecida
Barroso,Henrique de Almeida
Oliveira,Alaíde Braga de
author_role author
author2 Barroso,Henrique de Almeida
Oliveira,Alaíde Braga de
author2_role author
author
dc.contributor.author.fl_str_mv Takahashi,Jacqueline Aparecida
Barroso,Henrique de Almeida
Oliveira,Alaíde Braga de
dc.subject.por.fl_str_mv Cephalosporium aphidicola
biotransformation
diterpene
topic Cephalosporium aphidicola
biotransformation
diterpene
description Parameters for a more efficient biotransformation of diterpene-like compounds by the fungus Cephalosporium aphidicola were established by carrying out microscale feedings at several conditions. Experiments were guided by thin layer chromatography and gas chromatography analysis. It was observed that the substrate should be added in ethanol at concentrations between 15 to 30 mg per 100 ml of medium. The extraction of the product showed to be more efficient when carried out from both mycelia and broth and using ethylacetate as the extracting solvent. The experiment should be stopped six days after feeding the substrate to the fungus for the best product yield.
publishDate 2000
dc.date.none.fl_str_mv 2000-06-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200003
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000200003
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822000000200003
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.31 n.2 2000
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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