Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells
Autor(a) principal: | |
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Data de Publicação: | 2009 |
Outros Autores: | , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300036 |
Resumo: | The recognition of bacterial components on the intestinal epithelial cells occurs through the toll-like receptors and is followed by the induction of an effective innate immune response. We analyzed receptor expression and signaling pathways involved in activation of human colon adenocarcinoma cells after stimulation with porins and LPS of Shigella flexneri. We also analyzed the expression and production of some cytokines, of intercellular adhesion molecule-1, of antimicrobial peptides human ²-defensins, and of the inducible form of nitric oxide synthase. Our data demonstrate that TLR2 is involved in porin recognition, whereas TLR4 with MD2, is required for LPS recognition. |
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Brazilian Journal of Microbiology |
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Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cellsPorinsTLRsPAMPsShigella flexneriShigella flexneriThe recognition of bacterial components on the intestinal epithelial cells occurs through the toll-like receptors and is followed by the induction of an effective innate immune response. We analyzed receptor expression and signaling pathways involved in activation of human colon adenocarcinoma cells after stimulation with porins and LPS of Shigella flexneri. We also analyzed the expression and production of some cytokines, of intercellular adhesion molecule-1, of antimicrobial peptides human ²-defensins, and of the inducible form of nitric oxide synthase. Our data demonstrate that TLR2 is involved in porin recognition, whereas TLR4 with MD2, is required for LPS recognition.Sociedade Brasileira de Microbiologia2009-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300036Brazilian Journal of Microbiology v.40 n.3 2009reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822009000300036info:eu-repo/semantics/openAccessElena,GrimaldiGiovanna,DonnarummaBrunella,PerfettoAnna,De FilippisAlessandro,MelitoAntonietta,Tufano Mariaeng2009-08-18T00:00:00Zoai:scielo:S1517-83822009000300036Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2009-08-18T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
title |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
spellingShingle |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells Elena,Grimaldi Porins TLRs PAMPs Shigella flexneri Shigella flexneri |
title_short |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
title_full |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
title_fullStr |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
title_full_unstemmed |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
title_sort |
Proinflammatory signal transduction pathway induced by Shigella flexneri porins in caco-2 cells |
author |
Elena,Grimaldi |
author_facet |
Elena,Grimaldi Giovanna,Donnarumma Brunella,Perfetto Anna,De Filippis Alessandro,Melito Antonietta,Tufano Maria |
author_role |
author |
author2 |
Giovanna,Donnarumma Brunella,Perfetto Anna,De Filippis Alessandro,Melito Antonietta,Tufano Maria |
author2_role |
author author author author author |
dc.contributor.author.fl_str_mv |
Elena,Grimaldi Giovanna,Donnarumma Brunella,Perfetto Anna,De Filippis Alessandro,Melito Antonietta,Tufano Maria |
dc.subject.por.fl_str_mv |
Porins TLRs PAMPs Shigella flexneri Shigella flexneri |
topic |
Porins TLRs PAMPs Shigella flexneri Shigella flexneri |
description |
The recognition of bacterial components on the intestinal epithelial cells occurs through the toll-like receptors and is followed by the induction of an effective innate immune response. We analyzed receptor expression and signaling pathways involved in activation of human colon adenocarcinoma cells after stimulation with porins and LPS of Shigella flexneri. We also analyzed the expression and production of some cytokines, of intercellular adhesion molecule-1, of antimicrobial peptides human ²-defensins, and of the inducible form of nitric oxide synthase. Our data demonstrate that TLR2 is involved in porin recognition, whereas TLR4 with MD2, is required for LPS recognition. |
publishDate |
2009 |
dc.date.none.fl_str_mv |
2009-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300036 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822009000300036 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822009000300036 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.40 n.3 2009 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122202604437504 |