Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009 |
Resumo: | The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses. |
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Brazilian Journal of Microbiology |
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Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02Pleurotus eryngii SI-02exopolysaccharideextractionantioxidant activityin vitroThe extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.Sociedade Brasileira de Microbiologia2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009Brazilian Journal of Microbiology v.44 n.4 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013000400009info:eu-repo/semantics/openAccessSun,XinyiHao,LongMa,HuaLi,TongZheng,LanMa,ZhaoZhai,GuoyinWang,LiqinGao,ShanglongLiu,XiaonanJia,MengshiJia,Leeng2014-03-27T00:00:00Zoai:scielo:S1517-83822013000400009Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-03-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
title |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
spellingShingle |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 Sun,Xinyi Pleurotus eryngii SI-02 exopolysaccharide extraction antioxidant activity in vitro |
title_short |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
title_full |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
title_fullStr |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
title_full_unstemmed |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
title_sort |
Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02 |
author |
Sun,Xinyi |
author_facet |
Sun,Xinyi Hao,Long Ma,Hua Li,Tong Zheng,Lan Ma,Zhao Zhai,Guoyin Wang,Liqin Gao,Shanglong Liu,Xiaonan Jia,Mengshi Jia,Le |
author_role |
author |
author2 |
Hao,Long Ma,Hua Li,Tong Zheng,Lan Ma,Zhao Zhai,Guoyin Wang,Liqin Gao,Shanglong Liu,Xiaonan Jia,Mengshi Jia,Le |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Sun,Xinyi Hao,Long Ma,Hua Li,Tong Zheng,Lan Ma,Zhao Zhai,Guoyin Wang,Liqin Gao,Shanglong Liu,Xiaonan Jia,Mengshi Jia,Le |
dc.subject.por.fl_str_mv |
Pleurotus eryngii SI-02 exopolysaccharide extraction antioxidant activity in vitro |
topic |
Pleurotus eryngii SI-02 exopolysaccharide extraction antioxidant activity in vitro |
description |
The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-12-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822013000400009 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.4 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122205641113600 |