Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02

Detalhes bibliográficos
Autor(a) principal: Sun,Xinyi
Data de Publicação: 2013
Outros Autores: Hao,Long, Ma,Hua, Li,Tong, Zheng,Lan, Ma,Zhao, Zhai,Guoyin, Wang,Liqin, Gao,Shanglong, Liu,Xiaonan, Jia,Mengshi, Jia,Le
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009
Resumo: The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.
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spelling Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02Pleurotus eryngii SI-02exopolysaccharideextractionantioxidant activityin vitroThe extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.Sociedade Brasileira de Microbiologia2013-12-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009Brazilian Journal of Microbiology v.44 n.4 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013000400009info:eu-repo/semantics/openAccessSun,XinyiHao,LongMa,HuaLi,TongZheng,LanMa,ZhaoZhai,GuoyinWang,LiqinGao,ShanglongLiu,XiaonanJia,MengshiJia,Leeng2014-03-27T00:00:00Zoai:scielo:S1517-83822013000400009Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-03-27T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
title Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
spellingShingle Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
Sun,Xinyi
Pleurotus eryngii SI-02
exopolysaccharide
extraction
antioxidant activity
in vitro
title_short Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
title_full Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
title_fullStr Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
title_full_unstemmed Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
title_sort Extraction and in vitro antioxidant activity of exopolysaccharide by Pleurotus eryngii SI-02
author Sun,Xinyi
author_facet Sun,Xinyi
Hao,Long
Ma,Hua
Li,Tong
Zheng,Lan
Ma,Zhao
Zhai,Guoyin
Wang,Liqin
Gao,Shanglong
Liu,Xiaonan
Jia,Mengshi
Jia,Le
author_role author
author2 Hao,Long
Ma,Hua
Li,Tong
Zheng,Lan
Ma,Zhao
Zhai,Guoyin
Wang,Liqin
Gao,Shanglong
Liu,Xiaonan
Jia,Mengshi
Jia,Le
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Sun,Xinyi
Hao,Long
Ma,Hua
Li,Tong
Zheng,Lan
Ma,Zhao
Zhai,Guoyin
Wang,Liqin
Gao,Shanglong
Liu,Xiaonan
Jia,Mengshi
Jia,Le
dc.subject.por.fl_str_mv Pleurotus eryngii SI-02
exopolysaccharide
extraction
antioxidant activity
in vitro
topic Pleurotus eryngii SI-02
exopolysaccharide
extraction
antioxidant activity
in vitro
description The extraction parameters for Pleurotus eryngii SI-02 exopolysaccharide (EPS) produced during submerged culture were optimized using response surface methodology (RSM). The optimum conditions for EPS extraction were predicted to be, precipitation time 20.24 h, ethanol concentration 89.62% and pH 8.17, and EPS production was estimated at 7.27 g/L. The actual yield of EPS under these conditions was 7.21 g/L. The in vitro antioxidant results of the EPS showed that the inhibition effects of EPS at a dosage of 400 mg/L on hydroxyl, superoxide anion and 1,1-diphenyl-2-picrylhydrazyl (DPPH) radicals were 59.63 ± 3.72%, 38.69 ± 2.59%, and 66.36 ± 4.42%, respectively, which were 12.74 ± 1.03%, 8.01 ± 0.56%, and 12.19 ± 1.05% higher than that of butylated hydroxytoluene (BHT), respectively. The reducing power of EPS of P. eryngii SI-02 was 0.98 ± 0.05, 60.66 ± 5.14% higher than that of BHT. The results provide a reference for large-scale production of EPS by P. eryngii SI-02 in industrial fermentation and the EPS can be used as a potential antioxidant which enhances adaptive immune responses.
publishDate 2013
dc.date.none.fl_str_mv 2013-12-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000400009
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822013000400009
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.44 n.4 2013
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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