Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris

Detalhes bibliográficos
Autor(a) principal: Jiang,Guo-Feng
Data de Publicação: 2013
Outros Autores: Jiang,Bo-Le, Yang,Mei, Liu,San, Liu,Jiao, Liang,Xiao-Xia, Bai,Xian-Fang, Tang,Dong-Jie, Lu,Guang-Tao, He,Yong-Qiang, Yu,Di-Qiu, Tang,Ji-Liang
Tipo de documento: Artigo
Idioma: eng
Título da fonte: Brazilian Journal of Microbiology
Texto Completo: http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045
Resumo: It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc.
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spelling Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestrisinducing mediumtype III effector secretionXanthomonasIt is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc.Sociedade Brasileira de Microbiologia2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045Brazilian Journal of Microbiology v.44 n.3 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013000300045info:eu-repo/semantics/openAccessJiang,Guo-FengJiang,Bo-LeYang,MeiLiu,SanLiu,JiaoLiang,Xiao-XiaBai,Xian-FangTang,Dong-JieLu,Guang-TaoHe,Yong-QiangYu,Di-QiuTang,Ji-Liangeng2014-02-03T00:00:00Zoai:scielo:S1517-83822013000300045Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-02-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false
dc.title.none.fl_str_mv Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
title Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
spellingShingle Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
Jiang,Guo-Feng
inducing medium
type III effector secretion
Xanthomonas
title_short Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
title_full Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
title_fullStr Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
title_full_unstemmed Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
title_sort Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
author Jiang,Guo-Feng
author_facet Jiang,Guo-Feng
Jiang,Bo-Le
Yang,Mei
Liu,San
Liu,Jiao
Liang,Xiao-Xia
Bai,Xian-Fang
Tang,Dong-Jie
Lu,Guang-Tao
He,Yong-Qiang
Yu,Di-Qiu
Tang,Ji-Liang
author_role author
author2 Jiang,Bo-Le
Yang,Mei
Liu,San
Liu,Jiao
Liang,Xiao-Xia
Bai,Xian-Fang
Tang,Dong-Jie
Lu,Guang-Tao
He,Yong-Qiang
Yu,Di-Qiu
Tang,Ji-Liang
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.contributor.author.fl_str_mv Jiang,Guo-Feng
Jiang,Bo-Le
Yang,Mei
Liu,San
Liu,Jiao
Liang,Xiao-Xia
Bai,Xian-Fang
Tang,Dong-Jie
Lu,Guang-Tao
He,Yong-Qiang
Yu,Di-Qiu
Tang,Ji-Liang
dc.subject.por.fl_str_mv inducing medium
type III effector secretion
Xanthomonas
topic inducing medium
type III effector secretion
Xanthomonas
description It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc.
publishDate 2013
dc.date.none.fl_str_mv 2013-09-01
dc.type.driver.fl_str_mv info:eu-repo/semantics/article
dc.type.status.fl_str_mv info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.uri.fl_str_mv http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045
url http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045
dc.language.iso.fl_str_mv eng
language eng
dc.relation.none.fl_str_mv 10.1590/S1517-83822013000300045
dc.rights.driver.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv text/html
dc.publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
publisher.none.fl_str_mv Sociedade Brasileira de Microbiologia
dc.source.none.fl_str_mv Brazilian Journal of Microbiology v.44 n.3 2013
reponame:Brazilian Journal of Microbiology
instname:Sociedade Brasileira de Microbiologia (SBM)
instacron:SBM
instname_str Sociedade Brasileira de Microbiologia (SBM)
instacron_str SBM
institution SBM
reponame_str Brazilian Journal of Microbiology
collection Brazilian Journal of Microbiology
repository.name.fl_str_mv Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)
repository.mail.fl_str_mv bjm@sbmicrobiologia.org.br||mbmartin@usp.br
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