Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris
Autor(a) principal: | |
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Data de Publicação: | 2013 |
Outros Autores: | , , , , , , , , , , |
Tipo de documento: | Artigo |
Idioma: | eng |
Título da fonte: | Brazilian Journal of Microbiology |
Texto Completo: | http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045 |
Resumo: | It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc. |
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Brazilian Journal of Microbiology |
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Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestrisinducing mediumtype III effector secretionXanthomonasIt is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc.Sociedade Brasileira de Microbiologia2013-09-01info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersiontext/htmlhttp://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045Brazilian Journal of Microbiology v.44 n.3 2013reponame:Brazilian Journal of Microbiologyinstname:Sociedade Brasileira de Microbiologia (SBM)instacron:SBM10.1590/S1517-83822013000300045info:eu-repo/semantics/openAccessJiang,Guo-FengJiang,Bo-LeYang,MeiLiu,SanLiu,JiaoLiang,Xiao-XiaBai,Xian-FangTang,Dong-JieLu,Guang-TaoHe,Yong-QiangYu,Di-QiuTang,Ji-Liangeng2014-02-03T00:00:00Zoai:scielo:S1517-83822013000300045Revistahttps://www.scielo.br/j/bjm/ONGhttps://old.scielo.br/oai/scielo-oai.phpbjm@sbmicrobiologia.org.br||mbmartin@usp.br1678-44051517-8382opendoar:2014-02-03T00:00Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM)false |
dc.title.none.fl_str_mv |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
title |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
spellingShingle |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris Jiang,Guo-Feng inducing medium type III effector secretion Xanthomonas |
title_short |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
title_full |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
title_fullStr |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
title_full_unstemmed |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
title_sort |
Establishment of an inducing medium for type III effector secretion in Xanthomonas campestris pv. campestris |
author |
Jiang,Guo-Feng |
author_facet |
Jiang,Guo-Feng Jiang,Bo-Le Yang,Mei Liu,San Liu,Jiao Liang,Xiao-Xia Bai,Xian-Fang Tang,Dong-Jie Lu,Guang-Tao He,Yong-Qiang Yu,Di-Qiu Tang,Ji-Liang |
author_role |
author |
author2 |
Jiang,Bo-Le Yang,Mei Liu,San Liu,Jiao Liang,Xiao-Xia Bai,Xian-Fang Tang,Dong-Jie Lu,Guang-Tao He,Yong-Qiang Yu,Di-Qiu Tang,Ji-Liang |
author2_role |
author author author author author author author author author author author |
dc.contributor.author.fl_str_mv |
Jiang,Guo-Feng Jiang,Bo-Le Yang,Mei Liu,San Liu,Jiao Liang,Xiao-Xia Bai,Xian-Fang Tang,Dong-Jie Lu,Guang-Tao He,Yong-Qiang Yu,Di-Qiu Tang,Ji-Liang |
dc.subject.por.fl_str_mv |
inducing medium type III effector secretion Xanthomonas |
topic |
inducing medium type III effector secretion Xanthomonas |
description |
It is well known that the type III secretion system (T3SS) and type III (T3) effectors are essential for the pathogenicity of most bacterial phytopathogens and that the expression of T3SS and T3 effectors is suppressed in rich media but induced in minimal media and plants. To facilitate in-depth studies on T3SS and T3 effectors, it is crucial to establish a medium for T3 effector expression and secretion. Xanthomonas campestris pv. campestris (Xcc) is a model bacterium for studying plant-pathogen interactions. To date no medium for Xcc T3 effector secretion has been defined. Here, we compared four minimal media (MME, MMX, XVM2, and XOM2) which are reported for T3 expression induction in Xanthomonas spp. and found that MME is most efficient for expression and secretion of Xcc T3 effectors. By optimization of carbon and nitrogen sources and pH value based on MME, we established XCM1 medium, which is about 3 times stronger than MME for Xcc T3 effectors secretion. We further optimized the concentration of phosphate, calcium, and magnesium in XCM1 and found that XCM1 with a lower concentration of magnesium (renamed as XCM2) is about 10 times as efficient as XCM1 (meanwhile, about 30 times stronger than MME). Thus, we established an inducing medium XCM2 which is preferred for T3 effector secretion in Xcc. |
publishDate |
2013 |
dc.date.none.fl_str_mv |
2013-09-01 |
dc.type.driver.fl_str_mv |
info:eu-repo/semantics/article |
dc.type.status.fl_str_mv |
info:eu-repo/semantics/publishedVersion |
format |
article |
status_str |
publishedVersion |
dc.identifier.uri.fl_str_mv |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045 |
url |
http://old.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000300045 |
dc.language.iso.fl_str_mv |
eng |
language |
eng |
dc.relation.none.fl_str_mv |
10.1590/S1517-83822013000300045 |
dc.rights.driver.fl_str_mv |
info:eu-repo/semantics/openAccess |
eu_rights_str_mv |
openAccess |
dc.format.none.fl_str_mv |
text/html |
dc.publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
publisher.none.fl_str_mv |
Sociedade Brasileira de Microbiologia |
dc.source.none.fl_str_mv |
Brazilian Journal of Microbiology v.44 n.3 2013 reponame:Brazilian Journal of Microbiology instname:Sociedade Brasileira de Microbiologia (SBM) instacron:SBM |
instname_str |
Sociedade Brasileira de Microbiologia (SBM) |
instacron_str |
SBM |
institution |
SBM |
reponame_str |
Brazilian Journal of Microbiology |
collection |
Brazilian Journal of Microbiology |
repository.name.fl_str_mv |
Brazilian Journal of Microbiology - Sociedade Brasileira de Microbiologia (SBM) |
repository.mail.fl_str_mv |
bjm@sbmicrobiologia.org.br||mbmartin@usp.br |
_version_ |
1752122205610704896 |